⮝ Full datasets listing
PXD071273-1
PXD071273 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A repressor-activator bridging protein drives transcription factor binding to HDAC/Rpd3L |
| Description | Phenotypic characterization of members of Rpd3L demonstrate the importance of peripheral components including transcriptional repression and activation. To characterize these components, endogenous yeast Rpd3L complex was purified using three baits: Rxt3Tap, Sap30Tap, and Sin3Tap. Cryo-EM structures were acquired on each bait. Taking the Sap30Tap purification, enrichment and crosslinking analysis was performed using mass spectrometry. Integrating this data allowed for an integrated structural model to expand the structure from the cryo-EM core of Rpd3L to a complete model of Rpd3L including its incorporation of Ash1 and Cti6. Further integration of fragment-fragment interaction mapping and crystal structures at the interface of Sin3 PAH2 and Ume6 SID allowed for the incorporation of Ume6. A mutagenesis screen further verified specific residues that are essential for the interaction of Sin3 to Ume6 and Sin3 to Sap30. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-08-03 |
| AnnouncementXML | Submission_2026-08-03_14:45:12.144.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Joseph Cesare |
| SpeciesList | scientific name: Saccharomyces cerevisiae; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-26 10:39:35 | ID requested | |
| ⏵ 1 | 2026-08-03 14:45:12 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: XL-MS, cryo-EM, integrated structural modeling, epigenetics, transcription, histone deacetylation, DatasetType:Proteomics |
Contact List
| Michael P Washburn | |
|---|---|
| contact affiliation | Department of Cancer Biology University of Kansas Medical Center |
| contact email | mwashburn4@kumc.edu |
| lab head | |
| Joseph Cesare | |
| contact affiliation | University of Kansas School of Medicine |
| contact email | jcesare@kumc.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100035/ |
