⮝ Full datasets listing
PXD071203-1
PXD071203 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | CD4+ T cell surface biotinylation using pre-complexed Siglec-10 Fc and Protein A-APEX2 |
| Description | Follicular CD4+ T cells were enriched from pooled splenocytes in immunized C57BL/6J mice. 1 million cells per well in a 96-well V-bottom plate were incubated with 1X PBS containing 2 ug/mL Siglec-10 Fc (or Siglec-10 R119A Fc) pre-complexed with hemin-containing Protein A-APEX2 (1:2 molar ratio) for 30 min at RT, washed with 1X PBS and incubated with 500 uM biotin-tyramide (Millipore) in 1X PBS for 30 min at RT. Cells were then washed with 1X PBS and incubated with 500 uM H2O2 for 1 min at RT to initiate protein biotinylation. Reaction was quenched by washing cells 3 times with 1X PBS supplemented with 5 mM Trolox (Millipore) and 10 mM sodium ascorbate. Cell pellets were lysed in RIPA lysis buffer with protease inhibitors and underwent automated streptavidin beads enrichment (Pierce PI88817) using KingFisher Duo Prime and on-bead trypsin digestion. 10R: Sig-10 R119A Fc; 10W: Sig-10 WT Fc. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-11-25 |
| AnnouncementXML | Submission_2025-11-25_11:31:30.706.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Olivier Julien |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-25 11:20:40 | ID requested | |
| ⏵ 1 | 2025-11-25 11:31:31 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Siglec-G, B cells, APEX, Cell surface biotinylation, Proximity labeling, DatasetType:Proteomics |
Contact List
| Olivier Julien | |
|---|---|
| contact affiliation | University of Alberta |
| contact email | ojulien@ualberta.ca |
| lab head | |
| Matthew Macauley | |
| contact affiliation | University of Alberta |
| contact email | macauley@ualberta.ca |
| lab head | |
| Olivier Julien | |
| contact affiliation | University of Alberta |
| contact email | ojulien@ualberta.ca |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100018/ |
