PXD071198 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of salicylic acid (SA)- and pathogen-responsive ubiquitin-binding proteins in Arabidopsis thaliana |
| Description | The ubiquitin-proteasome system (UPS) is a key regulatory mechanism in plant immunity. Target proteins are labelled with ubiquitin chains that form distinct topological structures to modulate their activity and stability. Here, we identified ubiquitin-binding proteins (UBPs) that specifically interact with M1-, K48-, and K63-linked ubiquitin chains in response to SA or infection with the bacterial pathogen Pseudomonas syringae pv. maculicola ES4326 (Psm). Four-week-old plants were sprayed with 0.5 mM SA or inoculated with Psm ES4326, samples were harvested after 24 hours post-treatment. For the M1- and K48-linked ubiquitin chain pulldown assays, monoubiquitin (Ub) was used as a control. For the K63-linked ubiquitin chain pulldown, empty agarose beads (Beads) served as the control. In total three independent biological repeats were collected. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-12 |
| AnnouncementXML | Submission_2026-02-12_09:07:24.432.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zhishuo Wang |
| SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-25 09:03:59 | ID requested | |
| ⏵ 1 | 2026-02-12 09:07:24 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
Contact List
| Steven Spoel |
|---|
| contact affiliation | Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh |
| contact email | Steven.Spoel@ed.ac.uk |
| lab head | |
| Zhishuo Wang |
|---|
| contact affiliation | University of Edinburgh |
| contact email | zhishuo.wang@ed.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071198
- Label: PRIDE project
- Name: Identification of salicylic acid (SA)- and pathogen-responsive ubiquitin-binding proteins in Arabidopsis thaliana
