PXD071195-1

PXD071195 is an original dataset announced via ProteomeXchange.

Title	Altered growth and molecular features of Jurkat cells after introduction of the BCR-ABL1 p190 gene fusion
Description	The Philadelphia Chromosome is the result of a balanced reciprocal translocation be-tween the long arms of chromosomes 9 and 22, resulting in the fusion gene BCR-ABL1. Despite its being a hallmark of acute lymphocytic leukemia (ALL), acute myelogenous leukemia (AML) and mixed-phenotype acute leukemia, still comparatively little is known about effects that can be directly attributed to its presence in cancer cells. In order to study this question, we have created and characterised a Jurkat cell line with such an alteration, using a CRISPR/Cas9 based approach. The BCR-ABL1 p190 carrying cells we created showed increased proliferation as well as elevated sensitivity towards tyrosine kinase inhibitors as compared to unaltered Jurkat cells. By comparing gene expression, DNA-methylation and protein expression data, generated by NGS and mass spectrometry analyses, we were able to identify a number of pathways as well as individual proteins that show specific responses to the presence of BCR-ABL1 p190. Among the deregulated proteins we found known cancer proteins such as the tumor suppressors ASS1 and ABI3, which were downregulated in our model, or the specifically upregulated TRBC1. With an almost fivefold expression DPYSL5 showed the highest upregulation and PLEK was the protein with the strongest decrease (8.8 fold). Particularly noteworthy is the downregulation of CYP51A1 which is known to confer TKI-resistance under normal circumstances and is therefore directly in line with the increased TKI sensitivity of our BCR-ABL1 p190 positive cells. Also of interest is SPART, whose expression in our model was increased despite strong hypermethylation and it can be assumed that this paradoxical regulation might be directly attributable to the Philadelphia Chromosome.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_17:04:10.828.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Sura
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-25 07:45:21	ID requested
⏵ 1	2026-02-22 17:04:11	announced

Hossain MF, Hagenau L, Jensen LR, Rhode J, Sura T, Salazar MG, Tzvetkova A, Jensen C, Edwards S, Dunkel H, Simm S, Radke J, Kuss AW, Molecular background of Philadelphia chromosome dependent enhancement of cellular growth and tyrosine kinase inhibitor sensitivity. Exp Hematol Oncol, 15(1):26(2026) [pubmed]

10.1186/s40164-026-00758-4;

submitter keyword: Jurkat,BCR-ABL1, p190, ALL

Elke Hammer
contact affiliation	Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany
contact email	hammer@uni-greifswald.de
lab head
Thomas Sura
contact affiliation	University Medicine Greifswald, Interfaculty Institute for Genetics and Functional Genomics, Department of Functional Genomics
contact email	thomas.sura@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071195
     1. Label: PRIDE project
     2. Name: Altered growth and molecular features of Jurkat cells after introduction of the BCR-ABL1 p190 gene fusion