PXD071159-1

PXD071159 is an original dataset announced via ProteomeXchange.

Title	Physiology, functional genomics and proteomics of Verruconatronum alginilyticum gen. nov., sp. nov., a first haloalkaliphilic representative of the phylum Verrucomicrobiota from soda habitats
Description	Despite cultivation of many representatives of rich bacterial communities inhabiting alkaline soda lakes, members of the bacterial phylum Verrucomicrobiota have so far been detected only by molecular techniques. Here, we used alginate as a selective substrate to enrich and isolate two strains of haloalkaliphilic Verrucomicrobiota. The isolates share identical 16S rRNA gene sequences and represent a new genus, and, together with metagenome assembled genomes, a new family within Opitutales. Cells of strains AB-alg1T (from soda lakes) and AB-alg4 (from soda soils) are small motile cocci forming submerged colonies in soft alginate agar. They are saccharolytic heterotrophs growing aerobically on polysaccharides (alginate, starch and inulin) and sugars (glucose, fructose, mannose, sucrose, melezitose, maltose and cellobiose). They also grow anaerobically by fermentation of alginate and D-mannose and by coupling incomplete denitrification to oxidation of alginate. Both isolates are obligately alkaliphilic and moderately salt-tolerant. The dominant membrane phospholipids include phosphatidylcholine and cardiolipin. The genome of AB-alg1T features polysaccharide lyases of the PL6, 7, 15, 17, 38, and 39 families for depolymerization of alginate. On the basis of phenotypic and distinct phylogeny, we propose classification of strains AB-alg1T (JCM 35393T=UQM 41574T) and AB-alg4 as Verruconatronum alginilyticum gen. nov., sp. nov. within a new family Verruconatronumaceae.
HostingRepository	PRIDE
AnnounceDate	2026-04-17
AnnouncementXML	Submission_2026-04-17_13:21:52.765.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marc Strous
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-24 20:39:58	ID requested
⏵ 1	2026-04-17 13:21:53	announced

Dataset with its publication pending

submitter keyword: alginate, Verrucomicrobiota, alkaline soda lake

Marc Strous
contact affiliation	University of Calgary
contact email	mstrous@ucalgary.ca
lab head
Marc Strous
contact affiliation	University of Calgary
contact email	mstrous@ucalgary.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071159
     1. Label: PRIDE project
     2. Name: Physiology, functional genomics and proteomics of Verruconatronum alginilyticum gen. nov., sp. nov., a first haloalkaliphilic representative of the phylum Verrucomicrobiota from soda habitats