PXD071144 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A multidisciplinary integrated approach for the identification and characterization of the AMP profile in Hermetia illucens hemolymph |
| Description | The growing threat of antimicrobial resistance calls for alternative strategies to conventional antibiotics. Insects represent a promising source of antimicrobial peptides (AMPs) due to their potent innate immune responses. In this study, we investigated the peptide extracts from hemolymph of Hermetia illucens larvae as a bioresource of infection-induced AMPs. Larvae were challenged with Gram-negative (Escherichia coli) and Gram-positive (Micrococcus flavus) bacteria, and hemolymph-derived peptides were extracted and fractionated via reverse-phase high-performance liquid chromatography (RP-HPLC). Antibacterial activity of peptide fractions was evaluated in vitro against a panel of pathogenic strains, including multidrug-resistance isolated such as carbapenem-resistant Klebsiella pneumoniae. The peptide fractions were further analyzed by SDS-PAGE and LC-MS/MS to identify candidate AMPs. In parallel, shotgun proteomics on unfractionated peptide extracts enabled quantitative comparison across experimental conditions. In silico analysis using ProP 1.0, APD3, and CAMPR4 prediction tools revealed physicochemical profiles and machine learning-based scores consistent with antimicrobial function. Our integrative approach confirms that bacterial infection induces a diverse and bioactive immunopeptidome in H. illucens, supporting its potential as a sustainable platform for discovery of novel antimicrobial agents. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-01 |
| AnnouncementXML | Submission_2026-05-31_16:27:37.718.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | ilaria iacobucci |
| SpeciesList | scientific name: Hermetia illucens; NCBI TaxID: NEWT:343691; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 240 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-24 13:21:09 | ID requested | |
| ⏵ 1 | 2026-05-31 16:27:38 | announced | |
Publication List
| De Stefano F, Monaco V, Giglio F, Scieuzo C, Rinaldi R, Salvia R, Quaranta G, Amaro S, Fusco A, Mancini IM, Monti M, Donnarumma G, Falabella P, Hemolymph. Insects, 17(5):(2026) [pubmed] |
| 10.3390/insects17050486; |
Keyword List
| submitter keyword: Antimicrobial peptides |
| Hermetia illucens |
| Antimicrobial resistance |
| Proteomics |
| In vitro antibacterial assay |
| In silico prediction |
Contact List
| Maria Monti |
|---|
| contact affiliation | University of Naples Federico II and CEINGE Biotecnologie Avanzate Franco Salvatore |
| contact email | montimar@unina.it |
| lab head | |
| ilaria iacobucci |
|---|
| contact affiliation | University of Naples Federico II |
| contact email | ilaria.iacobucci@unina.it |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071144
- Label: PRIDE project
- Name: A multidisciplinary integrated approach for the identification and characterization of the AMP profile in Hermetia illucens hemolymph
