PXD071137-1

PXD071137 is an original dataset announced via ProteomeXchange.

Title	Chaperone-mediated autophagy regulates macrophage clearance of senescent cells
Description	Autophagy dysfunction and senescence are established drivers of aging, but their interplay remains poorly understood. Here, we identify how the age-related decline in chaperone-mediated autophagy (CMA) affects properties and fate of senescent cells in old organisms. Cells with genetically downregulated CMA, mimicking its aging decline, still undergo senescence but display a distinct senescence-associated secretory phenotype, and proteomic and metabolic profiles phenocopying those in old senescent cells. Combined CMA decline in senescent cells and macrophages impair senescent cell clearance. In fact, macrophage-specific CMA blockage in mice is sufficient for senescent cell accumulation, highlighting a key role for macrophage CMA in their recognition and removal. Conversely, pharmacological CMA activation reduces senescent cell burden in old mice and disease severity in a pulmonary fibrosis mouse model. Our findings reveal a functional interplay between CMA decline and senescence cell persistence, highlighting CMA upregulation as a promising strategy to promote senescence resolution in old organisms.
HostingRepository	PRIDE
AnnounceDate	2026-08-21
AnnouncementXML	Submission_2026-08-21_10:25:52.408.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simone Sidoli
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-24 10:47:19	ID requested
⏵ 1	2026-08-21 10:25:52	announced

Dataset with its publication pending

submitter keyword: Aging

Immune Clearance

Lysosomes

Proteostasis

Senescence

Simone Sidoli
contact affiliation	Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, United States
contact email	simone.sidoli@einsteinmed.edu
lab head
Simone Sidoli
contact affiliation	Albert Einstein College of Medicine
contact email	simone.sidoli@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD071137

PRIDE project URI

• PRIDE
  1. PXD071137
     1. Label: PRIDE project
     2. Name: Chaperone-mediated autophagy regulates macrophage clearance of senescent cells