PXD071116-1

PXD071116 is an original dataset announced via ProteomeXchange.

Title	Dual-Optimized Surface Hydrophobicity and Rigidity Determines Protein Corona Absorption and Composition on Orally Delivered Nanoparticles Treating Colitis
Description	Disease-specific protein corona adsorbed on nanocarriers critically determines their in vivo delivery efficiency. Given that macrophages can orchestrate inflammation resolution and mucosa repair, they are regarded as promising therapeutic targets in colitis. Here, we reveal that by altering the physicochemical properties of nanocarriers, we can manipulate the amount and composition of colitis-specific intestinal protein corona (C-IPC) specifically formed on orally administered nanoparticles (NPs), thereby improving colon macrophage targeting and colitis therapy. We demonstrate that increasing surface hydrophobicity of NPs boosts overall protein adsorption during C-IPC formation, leading to improved colon macrophage delivery and colitis therapeutic efficacy of high hydrophobic NPs (Phobic) loaded with budesonide. Moreover, further increasing the rigidity of Phobic can selectively enrich the corona with key macrophage-targeting related proteins, notably S100A8. This dual-optimized hydrophobicity-rigidity co-modulation approach generates an optimal C-IPC characterized by both high protein amount and a high proportion of targeting proteins. Benefiting from it, Phobic with high rigidity (Hard) further promoted the macrophage targeted drug delivery, thus more effectively attenuating the inflammation and restoring the immune homeostasis in male colitis rats. Our work develops a rational strategy of manipulating the disease-specific protein corona formation through multiple-physicochemical properties co-modulation for efficient drug delivery, holding broad promise for diverse nanocarriers and pathologies.
HostingRepository	PRIDE
AnnounceDate	2026-01-21
AnnouncementXML	Submission_2026-01-20_18:19:49.261.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenbo Cheng
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-24 00:25:47	ID requested
⏵ 1	2026-01-20 18:19:49	announced

Dataset with its publication pending

submitter keyword: Colitis, Rigidity, Hydrophobicity, Oral,Disease-specific protein corona

Mingjie Ni
contact affiliation	Sichuan University
contact email	mingjieni2022@163.com
lab head
Wenbo Cheng
contact affiliation	Shanghai Omicsspace Bioteh CO., LTD
contact email	wbcheng@omicsspace.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD071116

PRIDE project URI

• PRIDE
  1. PXD071116
     1. Label: PRIDE project
     2. Name: Dual-Optimized Surface Hydrophobicity and Rigidity Determines Protein Corona Absorption and Composition on Orally Delivered Nanoparticles Treating Colitis