PXD071112 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | 14-3-3θ Phosphorylation at S232 Reduces its Interactome and Regulates Axonal Trafficking |
| Description | 14-3-3 proteins impact protein-protein interactions (PPIs) that regulate neuronal functions. The 14-3-3θ isoform is protective in Parkinson’s disease (PD) and Dementia with Lewy Bodies (DLB) models. Human PD and DLB brains show increased 14-3-3θ phosphorylation at S232. To understand the impact of 14-3-3θ phosphorylation on brain PPIs, we performed affinity-purification mass spectrometry (AP-MS) using S232 phospho-mutant knock-in models. Proteins binding 14-3-3θ in Cre control cortical lysates were enriched in proteins involved in neuronal morphogenesis and microtubule dynamics. We found a dramatic decrease in proteins binding to 14-3-3θ in S232D mice compared to S232A mice. Axonal trafficking associated with these differentially binding proteins. Live imaging of acidic vesicles in axons revealed reduced net velocity in S232A and S232D neurons compared to Cre controls. In S232D neurons, this was due to a dramatic increase in vesicle pausing, while S232A neurons showed reduced segmental velocity, suggesting disrupted dynein motility. We conclude 14-3-3θ phosphorylation fine tunes axonal transport of acidic vesicles. Disruption of axonal transport with aberrant phosphorylation observed in PD and DLB could contribute to impaired clearance of aggregated proteins in these disorders. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-25 |
| AnnouncementXML | Submission_2025-11-24_17:07:50.497.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Talene Yacoubian |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-23 20:34:28 | ID requested | |
| ⏵ 1 | 2025-11-24 17:07:51 | announced | |
Publication List
| Pair FS, Pattanayak R, Mobley JA, Kojima K, Gannon M, Ekkatine R, Stone WJ, Scholz K, Yacoubian TA, phosphorylation at S232 reduces its interactome and regulates axonal trafficking. Dis Model Mech, 18(12):(2025) [pubmed] |
| 10.1242/dmm.052405; |
Keyword List
| submitter keyword: Axonal Trafficking, Affinity-purification Mass Spectrometry,14-3-3θ, Parkinson’s disease, Dementia with Lewy Bodies |
Contact List
| Talene Alene Yacoubian |
|---|
| contact affiliation | University of Alabama Birmingham Dept of Neurology |
| contact email | tyacoubian@uabmc.edu |
| lab head | |
| Talene Yacoubian |
|---|
| contact affiliation | University of Alabama at Birmingham |
| contact email | tyacoubian@uabmc.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071112
- Label: PRIDE project
- Name: 14-3-3θ Phosphorylation at S232 Reduces its Interactome and Regulates Axonal Trafficking
