PXD071097-1

PXD071097 is an original dataset announced via ProteomeXchange.

Title	LC-MSMS nucleoside analysis of Staphylococcus aureus RNA
Description	Staphylococcus aureus is a Gram-positive opportunistic pathogenic bacterium responsible for a broad spectrum of diseases, ranging from mild skin infections to life-threatening septicemia. Its remarkable capacity to withstand diverse and hostile environments, including antibiotic pressure and host immune defenses, poses major challenges for effective treatment. This adaptability stems from complex regulatory networks operating at multiple levels to fine-tune the expression of virulence factors and stress response pathways. Among these mechanisms, the role of post-transcriptional RNA modifications in ribosomal (rRNA) and transfer RNA (tRNA) remains largely underexplored, despite their potential impact on translation and global gene expression. These chemical alterations (e.g. methylation, deamination, isomerization) are introduced by dedicated enzymes, many of which respond to environmental cues, thereby modulating translation efficiency and fidelity and contributing to stress adaptation. The present data set provides LC-MS/MS-based quantification of RNA modification in S. aureus across diverse RNA samples.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-07_04:24:10.036.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jose Refugio Jaramillo Ponce
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: NEWT:1280;
ModificationList	No PTMs are included in the dataset
Instrument	6460 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-23 16:43:13	ID requested
⏵ 1	2026-09-07 04:24:12	announced

10.1093/nar/gkag757;

Jaramillo-Ponce JR, Wolff P, Marchand V, Motorin Y, Kohl M, Chane-Woon-Ming B, Kanazawa H, Ruiz-Paterson A, Paulus C, Chicher J, Lenon M, Kr, ä, mer M, Gribling-Burrer AS, Stathopoulos C, Smyth R, Helm M, Romby P, Marzi S, Comprehensive post-transcriptional modification profiles in individual Staphylococcus aureus tRNA species. Nucleic Acids Res, 54(14):(2026) [pubmed]

submitter keyword: LC-MSMS, RNA modifications,Staphylococcus aureus

Stefano Marzi
contact affiliation	UPR 9002 CNRS-ARN Université De Strasbourg IBMC
contact email	s.marzi@ibmc-cnrs.unistra.fr
lab head
Jose Refugio Jaramillo Ponce
contact affiliation	Université de Strasbourg, CNRS, Architecture et Réactivité de l’ARN, 67000 Strasbourg, France
contact email	j.jaramillo@ibmc-cnrs.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD071097

PRIDE project URI

• PRIDE
  1. PXD071097
     1. Label: PRIDE project
     2. Name: LC-MSMS nucleoside analysis of Staphylococcus aureus RNA