PXD071059-1

PXD071059 is an original dataset announced via ProteomeXchange.

Title	SpsB is a target of SOS-response inhibitor OXF‑077 and regulates quinolone resistance emergence
Description	Antimicrobial resistance (AMR) is a global health threat, urgently requiring novel compounds with new targets. One emerging strategy is the development of ‘antibiotic adjuvants’ that inhibit the mutagenic SOS response in bacteria, thus prolonging antibiotic efficacy. OXF‑077 is a potent SOS-response inhibitor that suppresses the emergence of ciprofloxacin resistance in Staphylococcus aureus; however, the cellular target of OXF-077 is unknown. We report here the use of affinity-based protein profiling to identify signal peptidase IB (SpsB) as a target of OXF‑077. Genetic and chemical studies demonstrated that SpsB is required for upregulation of the SOS response gene recA and increased frequency of resistance to ciprofloxacin. SpsB is therefore postulated to regulate the SOS response in S. aureus, representing a cell surface peptidase that can be targeted by small-molecules to slow the evolution of resistance. Collectively, this work delivers SpsB as an attractive target to combat the urgent threat of AMR.
HostingRepository	PRIDE
AnnounceDate	2026-05-21
AnnouncementXML	Submission_2026-05-21_06:16:25.966.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vaishnavi Ravikumar
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: NEWT:1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-21 14:24:56	ID requested
⏵ 1	2026-05-21 06:16:26	announced

10.1016/J.CHEMBIOL.2026.04.014;

submitter keyword: None

Thomas Lanyon-Hogg
contact affiliation	Department of Pharmacology, University of Oxford, United Kingdom
contact email	thomas.lanyon-hogg@pharm.ox.ac.uk
lab head
Vaishnavi Ravikumar
contact affiliation	Dept. of Biochemistry, University of Oxford
contact email	vaishnavi.ravikumar@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071059
     1. Label: PRIDE project
     2. Name: SpsB is a target of SOS-response inhibitor OXF‑077 and regulates quinolone resistance emergence