PXD070965-1

PXD070965 is an original dataset announced via ProteomeXchange.

Title	Plasmodium berghei zygotes of WT and Pbnek4-ko lines at 0 and 2 hpa phosphoproteomics
Description	Meiosis is a conserved yet evolutionarily varied process that underpins sexual reproduction in eukaryotes. In the malaria parasite Plasmodium, meiosis occurs within the mosquito midgut immediately after fertilisation, as the diploid zygote differentiates into a motile ookinete. Here, we identify the Plasmodium berghei NIMA-related kinase, NEK4 as a key regulator that coordinates meiotic initiation with zygote morphogenesis. Meiosis in Plasmodium spp. is highly divergent from that of most canonical models, since many conserved meiotic regulators are absent, and employs lineage-specific adaptations of protein function. Using gene tagging and ultrastructure expansion microscopy, we show that NEK4 is located at the microtubule-organising centre (MTOC) and the apical polar complex (APC) during early zygote development. NEK4-GFP foci appear shortly after fertilisation, followed by perinuclear and cortical microtubule formation. Deletion of nek4 results in complete developmental arrest: MTOC duplication and DNA replication are blocked, chromatin remains uncondensed, and nuclear migration and cell polarity fail to establish. Transcriptomic and phosphoproteomic analyses revealed that absence of NEK4 causes a collapse in transcriptional and phosphoregulatory networks controlling meiosis and cytoskeletal organisation. nek4 gene knockout parasites had reduced expression of genes linked to meiotic division, motility, and differentiation, and reduced phosphorylation of important players such as DMC1, AP2-Z, Pb103, HOP1, and REC8 proteins. These findings establish NEK4 as a key regulator governing transcriptional activation, protein synthesis, and phosphoregulation to drive meiotic entry and zygote maturation.
HostingRepository	PRIDE
AnnounceDate	2026-04-16
AnnouncementXML	Submission_2026-04-15_23:45:59.062.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD070965
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ryuji Yanase
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: NEWT:5823;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-19 17:44:32	ID requested
⏵ 1	2026-04-15 23:46:00	announced

10.21203/rs.3.rs-8180258/v1;

10.6019/PXD070965;

Yanase R, Hair M, Zeeshan M, Ferguson DJP, Brady D, Pasquarello C, Bottrill A, Bhanvadia S, Neal A, Tromer EC, Le Roch KG, Hainard A, Holder AA, Vaughan S, Guttery DS, Tewari R, NEK4 acts as a key regulator driving the early events of meiosis. Res Sq, ():(2025) [pubmed]

submitter keyword: Plasmodium berghei, NEK4, zygote, phosphoproteomics, meiosis

Rita Tewari
contact affiliation	School of Life Science, Queen's Medical Centre, University of Nottingham
contact email	rita.tewari@nottingham.ac.uk
lab head
Ryuji Yanase
contact affiliation	University of Nottingham
contact email	ryuji.yanase@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070965
     1. Label: PRIDE project
     2. Name: Plasmodium berghei zygotes of WT and Pbnek4-ko lines at 0 and 2 hpa phosphoproteomics