PXD070917 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Extracellular Vesicles from Adiponectin-Deficient Mice Mediate Skeletal Muscle Metabolic Dysfunction and Insulin Resistance |
| Description | Adiponectin regulates systemic metabolism and enhances extracellular vesicle (EV) biogenesis, but the impact of adiponectin deficiency on circulating EV composition and their impact on muscle metabolism remains unclear. Here, we integrated proteomic analyses of plasma EVs from wild-type (WT) and adiponectin-knockout (KO) mice fed chow or high-fat diet (HFD) with metabolomic profiling of quadriceps from these mice and functional analyses in skeletal muscle cells treated with these EVs. Plasma EVs showed altered size distribution and distinct proteomic signatures: KO-HFD EVs were depleted of mitochondrial and glycolytic enzymes (CS, MDH2, ALDOA, TPI1) that were enriched in WT-HFD EVs. We observed that KO EVs blunted insulin-stimulated Akt phosphorylation, glucose uptake, and mitochondrial respiration in L6 myotubes, whereas WT-HFD EVs improved these responses. Proteomic profiling of recipient cells revealed compensatory up-regulation of metabolic and stress-response pathways overlapping with those diminished in KO EVs, suggesting adaptive remodeling of cellular metabolism. Furthermore, quadricep muscle metabolomics revealed genotype- and diet-specific reductions in amino acid-derived intermediates of the tricarboxylic-acid (TCA) and urea cycles, indicating altered catabolic flux in KO mice. These findings identify an adiponectin-EV axis coordinating muscle metabolic homeostasis, offering insight into inter-organ metabolic communication under dietary stress. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-03 |
| AnnouncementXML | Submission_2026-08-03_10:51:09.377.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Vincent Richard |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-18 16:43:06 | ID requested | |
| ⏵ 1 | 2026-08-03 10:51:10 | announced | |
Publication List
| Lopez K, Lai SWT, Lopez Gonzalez EJ, D, á, vila RG, Shuck SC, Extracellular vesicles: A dive into their role in the tumor microenvironment and cancer progression. Front Cell Dev Biol, 11():1154576(2023) [pubmed] |
| 10.3389/fcell.2023.1154576; |
Keyword List
| submitter keyword: metabolism,adiponectin, proteomics, extracellular vesicles, skeletal muscle |
Contact List
| Christoph H Borchers |
|---|
| contact affiliation | Segal Cancer Proteomics Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University, Montreal, Québec, Canada |
| contact email | christoph.borchers@mcgill.ca |
| lab head | |
| Vincent Richard |
|---|
| contact affiliation | Lady Davis Institute for Medical Research, Jewish General Hospital, McGill University |
| contact email | vincentroyrichard@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD070917
- Label: PRIDE project
- Name: Extracellular Vesicles from Adiponectin-Deficient Mice Mediate Skeletal Muscle Metabolic Dysfunction and Insulin Resistance
