PXD070911 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | α7 Nicotinic Acetylcholine Receptor-Associated Signaling in U87MG Glioblastoma Cells (LFQ, Orbitrap Elite) |
| Description | This project investigates the role of α7 nicotinic acetylcholine receptor (α7 nAChR) signaling in glioblastoma using the U87MG human glioblastoma cell line. Cells were treated with selective α7 nAChR ligands (10 μM of agonist PNU282987, 1 μM of positive allosteric modulator PNU120596, 1 μM of antagonist α-cobratoxin) and their combination. Whole-cell lysates were processed for total proteome analysis, proteins were digested in-solution with trypsin and peptides analyzed by nano-ESI-LC-MS/MS on a Thermo Orbitrap Elite using DDA. Data were searched with PEAKS Studio 11 against the UniProtKB Human database and quantified by label-free quantification (LFQ). Proteomic changes in cytoskeletal, metabolic and ER-associated proteins were detected. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-09 |
| AnnouncementXML | Submission_2026-06-08_17:04:05.801.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD070911 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Elena Gondarenko |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-18 14:36:41 | ID requested | |
| ⏵ 1 | 2026-06-08 17:04:06 | announced | |
Publication List
Keyword List
| submitter keyword: glioblastoma |
| U87MG |
| α7 nicotinic acetylcholine receptor |
| choline |
| total proteome |
| label-free quantification |
| Orbitrap Elite |
| proteomics |
Contact List
| Igor Kasheverov |
|---|
| contact affiliation | Laboratory of Ligand-Receptor Interactions, Department of molecular neuroimmun signaling, Shemyakin-Ovchinnikov Institute of bioorganic chemistry of Russian academy of sciences, Moscow, Russian Federation |
| contact email | shak_ever@yahoo.com |
| lab head | |
| Elena Gondarenko |
|---|
| contact affiliation | IBCH RAS |
| contact email | gondarenkoea@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD070911 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD070911
- Label: PRIDE project
- Name: α7 Nicotinic Acetylcholine Receptor-Associated Signaling in U87MG Glioblastoma Cells (LFQ, Orbitrap Elite)
