PXD070852 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Structure of cytoplasmic RNA polymerase II |
| Description | Before RNA polymerase II (Pol II) can enter the nucleus, where it transcribes messenger RNA, it must first be assembled in the cytoplasm. We have performed proteomic and structural analysis of endogenous human cytoplasmic Pol II complexes, enriched in known biogenesis factors including RPAP2 and the essential small GTPases, GPN1, and GPN3. Cryo-EM analysis revealed a structure of Pol II bound to RPAP2 and Gdown1 at a nominal resolution of 2.7 Å, in which previously unresolved regions of Gdown1 could be modeled. Analysis of reconstituted complexes revealed the interactions responsible for Pol II-RPAP2 association with GPN1 and GPN3, as well as how GPN1 and GPN3 form an intricate assembly together with RPAP2, whose stability is affected by GTP hydrolysis state. These combined revealed a network of interactions that chaperone Pol II in the cytoplasm and allow recycling of critical biogenesis complexes, and suggest a general model for how GPN-loop GTPases function. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-14 |
| AnnouncementXML | Submission_2026-07-13_22:45:14.888.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Benjamin Neuditschko |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-17 12:11:59 | ID requested | |
| ⏵ 1 | 2026-07-13 22:45:15 | announced | |
Publication List
Keyword List
| submitter keyword: RPAP2, XL-MS, GPN1. GPN3, Gdown1,RNA polymerase II |
Contact List
| Franz Herzog |
|---|
| contact affiliation | Institute Krems Bioanalytics, IMC University of Applied Sciences, 3500, Krems, Austria |
| contact email | franz.herzog@imc.ac.at |
| lab head | |
| Benjamin Neuditschko |
|---|
| contact affiliation | Institute Krems Bioanalytics, IMC University of Applied Sciences Krems, 3500 Krems, Austria |
| contact email | benjamin.neuditschko@fh-krems.ac.at |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD070852
- Label: PRIDE project
- Name: Structure of cytoplasmic RNA polymerase II
