PXD070796 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Bacterial effector modifies host messenger RNA to promote virulence |
| Description | Bacterial pathogens harbor specialized secretion systems that inject effector proteins into the host cell to
establish infection and disease. While many bacterial effectors post-translationally modify proteins to influence
host responses, the extent to which effectors modify host RNA is currently unknown. Here we performed
RNA-interactome capture (RIC) to isolate effectors bound to host cellular messenger RNA (mRNA)
during Legionella pneumophila infection. RIC identified an uncharacterized effector, FadA (Lpw16921), which
localized to the host-cell nucleus and interacted with host mRNAs at uracil (U)-rich RNA motifs. FadA exhibited
NADPH-oxidase activity that mediated 8-oxo-guanine (oxo8G) modifications of mRNA substrates, resulting
in oxidative damage and inhibition of translation. Infection with L. pneumophila harboring wild-type
FadA, but not a catalytically inactive mutant, increased oxo8G modifications, suppressed cytokine responses,
and promoted bacterial persistence in vivo. Our findings demonstrate the potential for a secreted
Q2 effector to post-transcriptionally modify host mRNA as a mechanism to promote bacterial virulence. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-15 |
| AnnouncementXML | Submission_2026-08-14_19:30:43.943.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Kathleen McCaffrey |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-16 20:10:15 | ID requested | |
| 1 | 2026-08-14 03:06:49 | announced | |
| ⏵ 2 | 2026-08-14 19:30:44 | announced | 2026-08-15: Updated project metadata. |
Publication List
Keyword List
Contact List
| Kathleen McCaffrey |
|---|
| contact affiliation | Hudson Institute of Medical Research, Australia |
| contact email | kathleen.mccaffrey@hudson.org.au |
| lab head | |
| Kathleen McCaffrey |
|---|
| contact affiliation | Hudson Institute of Medical Research, Clayton, Victoria |
| contact email | kathleen.mccaffrey@hudson.org.au |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD070796 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD070796
- Label: PRIDE project
- Name: Bacterial effector modifies host messenger RNA to promote virulence
