PXD070795-1

PXD070795 is an original dataset announced via ProteomeXchange.

Title	Integrated Data-Independent Acquisition and Thermal Proteome Profiling for Proteomic Characterization of Lamotrigine-Treated MCF-7 Cells
Description	Lamotrigine, a clinically approved anticonvulsant, has recently gained interest for its potential anticancer effects. However, the molecular mechanisms underlying its activity in breast cancer remain poorly understood. Here, an integrated mass spectrometry-based proteomic strategy combining data-independent acquisition (DIA) and thermal proteome profiling (TPP) was applied to investigate the cellular effects and potential targets of lamotrigine in MCF-7 breast cancer cells. DIA-based quantitative approach identified 6,622 proteins, of which 142 were significantly modulated following lamotrigine exposure. Differentially expressed proteins were mainly associated with mitochondrial energy metabolism, chromatin organization, and protein translation. TPP experiments identified significant thermal stability alterations in ABRAXAS2, MT-CYB, TMEM97, and MTA2 upon lamotrigine treatment. Notably, both DIA and TPP analyses highlighted mitochondrial alterations, with consistent involvement of MT-CYB, suggesting mitochondrial dysfunction as a potential central mechanism of lamotrigine’s action. These findings support further investigation into its therapeutic potential and mechanism of action in breast cancer.
HostingRepository	PRIDE
AnnounceDate	2026-06-30
AnnouncementXML	Submission_2026-06-30_07:13:21.296.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Annarita Giuliano
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Exploris 480; Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-16 20:01:41	ID requested
⏵ 1	2026-06-30 07:13:21	announced

10.1007/S00216-026-06540-Z;

submitter keyword: breast cancer, proteomics,lamotrigine, thermal proteome profiling (TPP), data-independent acquisition (DIA), drug repurposing

Annarita Giuliano
contact affiliation	University "Magna Graecia" of Catanzaro
contact email	annarita.giuliano@studenti.unicz.it
lab head
Annarita Giuliano
contact affiliation	University of catanzaro
contact email	annarita.giuliano@studenti.unicz.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070795
     1. Label: PRIDE project
     2. Name: Integrated Data-Independent Acquisition and Thermal Proteome Profiling for Proteomic Characterization of Lamotrigine-Treated MCF-7 Cells