PXD070770-1

PXD070770 is an original dataset announced via ProteomeXchange.

Title	Chemical-proteomics profiling of reactive cysteines near a hydrophobic pocket
Description	This project investigates reactive cysteine residues located near hydrophobic pockets in proteins, features conserved in autopalmitoylated proteins. S-palmitoylation is a post-translational modification that regulates protein localization and function, and has been implicated in neurological, metabolic, infectious, and cancer-related processes. Autopalmitoylation occurs through a thioester transfer from palmitoyl-CoA to nucleophilic cysteine residues, a reaction that can be facilitated by structural or adaptor proteins with enzyme-like activities. In this study, chemical-proteomics approaches were applied to map reactive cysteines positioned adjacent to hydrophobic pockets that may serve as ligandable sites. These datasets enable the identification of druggable microenvironments in autopalmitoylated or other lipid-modified proteins, providing a resource for studying the mechanistic basis and therapeutic potential of palmitoylation-dependent regulation.
HostingRepository	PRIDE
AnnounceDate	2025-11-20
AnnouncementXML	Submission_2025-11-20_08:24:49.609.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD070770
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lu Hu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-14 22:08:30	ID requested
⏵ 1	2025-11-20 08:24:50	announced

10.6019/PXD070770;

submitter keyword: autopalmitoylation, hydrophobic pockets, chemical proteomics,reactive cysteines, druggable sites

Xu Wu
contact affiliation	Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, United States
contact email	xuwu@mgh.harvard.edu
lab head
Lu Hu
contact affiliation	Massachusetts General Hospital/Harvard Medical School
contact email	lhu8@mgh.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070770
     1. Label: PRIDE project
     2. Name: Chemical-proteomics profiling of reactive cysteines near a hydrophobic pocket