PXD070746-1

PXD070746 is an original dataset announced via ProteomeXchange.

Title	Theileria parva Muguga (TpM) schizonts LC-MS/MS
Description	The apicomplexan parasite Theileria parva (Tp) causes East Coast Fever (ECF), an often fatal tick-borne disease especially for Bos taurus cattle breeds that causes substantial economic losses for cattle farmers in sub-Saharan Africa. Replacement of the currently employed live vaccine with a more standardised, stable, affordable, and cross-protective subunit vaccine has not yet been successful and requires the identification of additional suitable parasite antigens. Carbohydrate-based molecules play an important role in the survival and establishment of pathogens including protozoan parasites and can represent strong virulence factors. The current knowledge of the glycobiology of Tp, however, is very limited which led us to investigate the glycosylation potential of Tp in this study. Screening of isolated Tp Muguga (TpM) schizonts with C-type lectin (-like) receptors identified recognition of the parasites by ruminant but not murine Macrophage C-type lectin (MCL). This suggested the presence of carbohydrate-associated schizont surface molecules. Specific binding of isolated schizonts by WGA but not ConA further suggested the presence of terminal GlcNAc residues on the surface of the parasites which is in alignment with the identification of a minimal N-glycosylation machinery in TpM schizonts. LC-MS/MS analyses of either HILIC-HPLC or WGA enriched peptides from parasite protein fractions identified eight predicted N-glycopeptides and two predicted O-glycopeptides. While putative parasite O-glycans were assigned with high confidence, assignments of putative N-glycans to parasite proteins were less confident. Confirmation of both the assignment and the composition of predicted parasite glycans requires further analyses.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-28_16:31:44.306.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shi Yan
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913; scientific name: Theileria parva; NCBI TaxID: NEWT:5875;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-14 09:44:56	ID requested
⏵ 1	2026-06-28 16:31:45	announced

10.3389/fvets.2026.1816563;

Kolakowski J, Yan S, Stadlmann J, Lepenies B, Ngugi D, Harris H, Werling D, parasites. Front Vet Sci, 13():1816563(2026) [pubmed]

submitter keyword: None

Shi Yan
contact affiliation	University of Veterinary Medicine Vienna
contact email	shi.yan@vetmeduni.ac.at
lab head
Shi Yan
contact affiliation	University of Veterinary Medicine Vienna
contact email	shi.yan@vetmeduni.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070746
     1. Label: PRIDE project
     2. Name: Theileria parva Muguga (TpM) schizonts LC-MS/MS