PXD070738-1

PXD070738 is an original dataset announced via ProteomeXchange.

Title	Vertical Stratification of P Pools in Subtropical Plantation Soils under Fertilization and Dry–Season Irrigation: Multiomics Regulatory Strategies
Description	The rapid expansion of fast-growing plantations in subtropical regions is closely linked to dry-season irrigation and fertilization; however, improper practices often lead to soil acidification and reduced nutrient bioavailability. Phosphorus (P), one of the most critical elements for plantation tree growth, shows complex spatial distribution patterns in soil that are influenced by multiple factors, directly affecting plantation productivity. This study investigated the effects of long-term fertilization and dry-season irrigation on the vertical distribution of phosphorus in an 8-year-old subtropical Eucalyptus plantation. This study employed stratified sampling (0–30 cm topsoil, 30–60 cm subsoil, 60–90 cm substratum) during dry seasons, coupled with metagenomics, metabolomics, and environmental factor analysis, to reveal vertical phosphorus cycling patterns and multiomics regulatory networks. Key findings: (1) Fertilization and dry-season irrigation had a limited influence on labile phosphorus and the diversity of P-cycling microorganisms. The topsoil presented significantly greater P availability than did the subsoil, manifested as elevated acid phosphatase activity (ACP), significant enrichment of the tryptophan metabolic pathway, and greater microbial diversity. (2) pH and the C:P ratio represent critical factors of vertical stratification in soil P cycling. Under acidic conditions, topsoil microorganisms facilitate P release via diverse metabolic pathways, whereas oligotrophic constraints in the substratum limit enzymatic activities. (3) We believe that potential cross-stratum microbial functional coordination exists in acidic soil P cycling, with linkages to tryptophan metabolism and polyP synthesis/degradation. Our study provides theoretical multiomics insights for optimizing the management of soil P pools in subtropical plantations under fertilization and dry-season irrigation.
HostingRepository	PRIDE
AnnounceDate	2025-12-09
AnnouncementXML	Submission_2025-12-09_09:24:02.832.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shitao Zhang
SpeciesList	scientific name: soil metagenome; NCBI TaxID: NEWT:410658;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-14 08:15:30	ID requested
⏵ 1	2025-12-09 09:24:03	announced

10.3389/FMICB.2025.1714023;

submitter keyword: ferralsols, multiomics, phosphorus cycling, vertical differentiation, plantation

Zhang Shitao
contact affiliation	Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
contact email	18282710227@163.com
lab head
Shitao Zhang
contact affiliation	Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
contact email	18282710227@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070738
     1. Label: PRIDE project
     2. Name: Vertical Stratification of P Pools in Subtropical Plantation Soils under Fertilization and Dry–Season Irrigation: Multiomics Regulatory Strategies