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PXD070732-1

PXD070732 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIn vivo protein N-terminal sequence analysis of WAGO-1 and WAGO-3 in C. elegans
DescriptionA mass spectrometry-based label-free proteomics analysis was performed to study the N-terminal protein sequences of WAGO-1 and WAGO-3 in C. elegans. Argonaute-enriched proteins were digested by trypsin. Peptides were measured on an Orbitrap Astral mass spectrometer. Raw data files were processed by MaxQuant software package (version 2.6.2.0) using its integrated Andromeda search engine. Spectral data were searched against a target-decoy database consisting of the forward and reverse sequences of the WormPep release WS292 (28,587 entries), the UniProt E. coli K-12 reference proteome (release 2022_03; 4,460 entries), a list of N-terminally truncated WAGO-1/WAGO-3 proteins, WAGO-3 (P2G, A3E) mutant and a default list of common contaminants. False discovery rate (FDR) was set to 1% at both peptide and protein levels.
HostingRepositoryMassIVE
AnnounceDate2026-09-08
AnnouncementXMLSubmission_2026-09-08_02:50:58.835.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJ Chen
SpeciesList scientific name: Caenorhabditis elegans; common name: roundworm; NCBI TaxID: 6239;
ModificationListiodoacetamide - site C
InstrumentOrbitrap Astral
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-11-14 06:02:31ID requested
12026-09-08 02:50:59announced
Publication List
no publication
Keyword List
submitter keyword: C. elegans, argonaute, N-terminus, WAGO-1, WAGO-3, DatasetType:Proteomics
Contact List
Rene Ketting
contact affiliationInstitute of Molecular Biology, Mainz
contact emailR.Ketting@imb-mainz.de
lab head
J Chen
contact affiliationInstitute of Molecular Biology
contact emailj.chen@imb-mainz.de
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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