⮝ Full datasets listing
PXD070677-1
PXD070677 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Rules of Coexistence: RiPP Recognition Elements Evolved to Prevent Pathway Interference through Leader Peptide Discrimination |
| Description | Identification of the precursor peptide post translational modifications by MALDI-TOF MS followed by Mascot search. LppK kinase modifies conserved Ser3 residue in LppA. LppA peptide fused with N-terminal MBP and C-terminal TrxA mass tag (MW 60.1 kDa) co-expressed with LppB harboring N-terminal Strep-tag and LppK. The LppB-LppA complex was purified using affinity chromatography. Protein zones corresponding to LppA (MBP-LppA-TrxA fused protein, MW 61.5 kDa) were subjected to in-gel digestion with trypsin. The eluted tryptic peptides were identified using MALDI-TOF MS. For the leader peptide cleavage site identification, LppA peptide fused with N-terminal MBP and C-terminal TrxA mass tag (MW 60.1 kDa) co-expressed with LppB harboring N-terminal Strep-tag and LppB2. The LppB-LppA complex was purified using affinity chromatography. Protein zones corresponding to LppA (MBP-LppA-TrxA fused protein, MW 61.5 kDa) were subjected to in-gel digestion with trypsin. The eluted tryptic peptides were identified using MALDI-TOF MS. |
| HostingRepository | jPOST |
| AnnounceDate | 2025-11-13 |
| AnnouncementXML | Submission_2025-11-13_03:28:33.208.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Aleksandr Popov |
| SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
| ModificationList | unknown modification; L-methionine sulfoxide; S-carboxamidoethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; S-carboxamidomethyl-L-cysteine |
| Instrument | ultraflex |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-13 00:01:24 | ID requested | |
| ⏵ 1 | 2025-11-13 03:28:33 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: RiPPs, kinase, peptide, peptidase, polyphosphorylation |
Contact List
| Svetlana Dubiley | |
|---|---|
| lab head | |
| Aleksandr Popov | |
| contact affiliation | RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa, 230-0045, Japan |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004185/ |
