PXD070642-2

PXD070642 is an original dataset announced via ProteomeXchange.

Title	Spore inoculum size impacts substrate degradation and sporulation but not the secretome during colonization of whole yellow pea (Pisum sativum) by Aspergillus oryzae
Description	Aspergillus oryzae is used in solid-state fermentation (SSF) to produce plant-based foods. To this end, the substrate is inoculated with spores of this fungus. So far, the effect of inoculum size on SSF with A. oryzae has primarily focused on the production of specific enzymes. Therefore, the aim of this study was to examine the impact of inoculum size on the full secretome, combined with enzyme activity assays, assessment of colonization, substrate degradation, and sporulation. To this end, A. oryzae was grown for 7 days on whole yellow pea (Pisum sativum). Fluorescence microscopy with a GFP-expressing A. oryzae strain showed that peas had been colonized externally and internally, irrespective of inoculum size. Yet, the highest inoculum size resulted in a stronger pea biomass reduction when compared to the lowest inoculum size. By contrast, sporulation decreased with increasing inoculum size. Notably, proteomics revealed no effect of inoculum size on the protein profiles of aqueous extracts of the colonized peas. Amylases and proteases were the most abundant secreted proteins, which was consistent with their high activity in the aqueous extracts. Proteomics also identified β-1,3-glucanases and chitinases, indicating hyphal lysis. Indeed, 10–19% of the fungal proteins detected in the aqueous extracts lacked signal peptides. These data contribute to our understanding of colonization of substrates by A. oryzae and may be used to optimize SSF with this food grade fungus.
HostingRepository	PRIDE
AnnounceDate	2026-06-27
AnnouncementXML	Submission_2026-06-27_07:41:29.671.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD070642
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nadzeya Staliarova
SpeciesList	scientific name: Pisum sativum (Garden pea); NCBI TaxID: NEWT:3888; scientific name: Aspergillus oryzae RIB40; NCBI TaxID: NEWT:510516;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-12 10:30:52	ID requested
1	2026-06-26 06:32:52	announced
⏵ 2	2026-06-27 07:41:30	announced	2026-06-27: Updated project metadata.

10.6019/PXD070642;

Braat M, Braat N, Staliarova N, Vellozo-Echevarr, í, a T, Siwek HM, Kemmer JF, Barrett K, Meyer AS, Stecker KE, W, ö, sten HAB, Spore inoculum size impacts substrate degradation and sporulation but not the secretome during colonization of whole yellow pea (Pisum sativum) by Aspergillus oryzae. Int J Food Microbiol, 458():111853(2026) [pubmed]

10.1016/j.ijfoodmicro.2026.111853;

submitter keyword: Fungus, solid-state fermentation, plant-based meat alternative, Aspergillus oryzae, Pisum sativum, pea

Kelly E. Stecker
contact affiliation	Biomolecular Mass Spectrometry and Proteomics, Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CH Utrecht, the Netherlands
contact email	k.e.stecker@uu.nl
lab head
Nadzeya Staliarova
contact affiliation	Utrecht University
contact email	n.staliarova@uu.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070642
     1. Label: PRIDE project
     2. Name: Spore inoculum size impacts substrate degradation and sporulation but not the secretome during colonization of whole yellow pea (Pisum sativum) by Aspergillus oryzae