PXD070623-1

PXD070623 is an original dataset announced via ProteomeXchange.

Title	Characterizations of G-quadruplex RNA-Protein Interactions in Living Cells
Description	RNA guanine quadruplexes (rG4s) are non-canonical nucleic acid structures essential for various cellular functions and disease pathways. Characterizing rG4-interacting proteins (rG4IPs) is crucial for deciphering biological roles of rG4s. Building upon the RNA-protein interaction detection (RaPID) method, we introduced G4-RaPID – a tailored strategy for robustly profiling rG4IPs in living cells. Using G4-RaPID with three distinct rG4 sequences in live cells, we identified 105 candidate rG4IPs with diverse biological functions. We also validated the direct binding of recombinant hnRNPA0, CHD4, and IGF2BP1 proteins with rG4 structures in vitro. Additionally, our CLIP-Seq data revealed an enrichment of hnRNPA0 at rG4 loci. Moreover, luciferase reporter assay results showed that hnRNPA0 interacts with the rG4 in the 5UTR of NRAS mRNA to negatively regulate its translation. Together, we developed an rG4-RaPID method for characterizing rG4-protein interactions in living cells, and documented the functions of hnRNPA0-rG4 interaction in modulating the translation of NRAS mRNA.
HostingRepository	PRIDE
AnnounceDate	2026-06-01
AnnouncementXML	Submission_2026-05-31_16:45:23.988.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	feng tang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-11 20:47:49	ID requested
⏵ 1	2026-05-31 16:45:24	announced

Tang F, Liang X, Porter DF, Miao W, Maehlmann K, Yuan J, Khavari PA, Wang Y, Characterizations of G-Quadruplex RNA-Protein Interactions in Living Cells. Anal Chem, 98(16):12090-12098(2026) [pubmed]

10.1021/acs.analchem.6c00902;

submitter keyword: G-quadruplex, RNA-Protein Interactions, Proteomics, LC-MS/MS

Yinsheng Wang
contact affiliation	1Department of Chemistry, University of California, Riverside
contact email	yinsheng@ucr.edu
lab head
feng tang
contact affiliation	University of California, riverside
contact email	fengt@ucr.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD070623

PRIDE project URI

• PRIDE
  1. PXD070623
     1. Label: PRIDE project
     2. Name: Characterizations of G-quadruplex RNA-Protein Interactions in Living Cells