PXD070546 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic characterization of human iPSC-derived cardiomyocytes |
| Description | Cardiomyocytes differentiated in vitro from human induced pluripotent stem cells (iPSC-CMs) are increasingly used in studies of disease mechanisms, drug development, toxicity testing, and regenerative medicine. However, the extent to which iPSC-CMs recapitulate native cardiac gene expression programs, at both the splicing and protein levels, remains poorly understood. We investigated the alternative splicing profiles of in vitro differentiated iPSC-CMs in comparison to fetal and adult heart samples. To examine whether splicing isoforms identified in RNA-seq datasets give rise to proteins, we generated a comprehensive proteomic dataset of iPSC-CMs. Integration of RNA and protein datasets enabled the detection of isoform-specific peptides, demonstrating translation of selected alternatively spliced transcripts. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-30 |
| AnnouncementXML | Submission_2025-12-30_03:26:12.308.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD070546 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | André Ventura-Gomes |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-10 08:03:56 | ID requested | |
| ⏵ 1 | 2025-12-30 03:26:12 | announced | |
Publication List
Keyword List
Contact List
| Carmo Fonseca |
|---|
| contact affiliation | GIMM – Gulbenkian Institute for Molecular Medicine |
| contact email | carmo.fonseca@gimm.pt |
| lab head | |
| André Ventura-Gomes |
|---|
| contact affiliation | GIMM – Gulbenkian Institute for Molecular Medicine |
| contact email | andre.v.gomes@gimm.pt |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD070546 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD070546
- Label: PRIDE project
- Name: Proteomic characterization of human iPSC-derived cardiomyocytes
