PXD070541-1

PXD070541 is an original dataset announced via ProteomeXchange.

Title	Salivary proteomics and metabolic responses to resistance training with and without blood flow restriction in young adults
Description	Partial oxygen restriction in muscles during exercise, known as blood flow restriction (BFR), has been applied as alternative strategy to reproduce the effects of conventional high-load intensity training (HI) while training with low-loads (LI). Saliva may serve as a reliable tool for understanding the biological pathways involved in both protocols. This intervention and cross-over study evaluated the metabolic responses and the salivary protein profile before and after two different resistance training protocols. Ten participants were selected and underwent to training with LI (30%1RM – one repetition maximum) plus BFR vs. HI (70%1RM) without BFR, at 48h-interval. Stimulated saliva was collected before and immediately after the sessions, while breath-by-breath oxygen uptake (VO2) was measured during and after each session. Arterial blood samples for lactate concentration measurement (in EqO2[La]) were taken at 1st minute of resting between each exercise of the protocols. For HI, there was an increase in two actin cytoplasmic isoforms and two immunoglobulin isoforms and a decrease of six hemoglobin isoforms. For BFR, there was an increase in two hemoglobin isoforms, and the same immunoglobulin isoforms (t-test, p < 0.05). No differences were significant between HI and LI+BRF training regarding the total energy demand (in mlO2), absolute oxygen values (mlO2) for oxidative response (VO2ON), glycolytic (EqO2[La]), and oxygen debt (VO2OFF) (p>0.05). Both HI and LI+BFR protocols modulated immune system activity and exhibited divergent hemoglobin patterns. While HI training predominantly upregulated proteins associated with energy metabolism and muscle remodeling, BFR training revealed a proteomic signature suggestive of neuroprotective effects.
HostingRepository	PRIDE
AnnounceDate	2025-12-26
AnnouncementXML	Submission_2025-12-26_04:30:04.361.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karina Santos
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Xevo G2 Q-Tof; nanoACQUITY UPLC

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-10 07:00:50	ID requested
⏵ 1	2025-12-26 04:30:04	announced

10.1016/J.JPROT.2025.105587;

submitter keyword: Oxygen Uptake,Biomarkers, Blood Lactate, Proteomics, Saliva., Resistance Training

Karina Oliveira Santos
contact affiliation	Universidade de São Paulo, Faculdade de Odontologia de Bauru
contact email	karina_oliveira@usp.br
lab head
Karina Santos
contact affiliation	Universidade de São Paulo, Faculdade de Odontologia de Bauru (FOB-USP)
contact email	karina_oliveira@usp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070541
     1. Label: PRIDE project
     2. Name: Salivary proteomics and metabolic responses to resistance training with and without blood flow restriction in young adults