PXD070506-1

PXD070506 is an original dataset announced via ProteomeXchange.

Title	Porphyromonas gingivalis bundled fimbriae interact with outer membrane vesicles, commensals and fibroblasts
Description	Porphyromonas gingivalis plays a key role in periodontal disease and has been associated with several serious systemic diseases. Its fimbriae are a major virulence factor. We re-cently demonstrated the formation of bundles of long FimA fimbriae in strain ATCC 33277. Transmission (TEM) and scanning electron microscopy (SEM) were used to exam-ine a collection of P. gingivalis strains representing all seven known FimA genotypes (I, Ib, IIa, IIb, III–V) and a P. gulae strain (type A). Additionally, two P. gingivalis strains (ATCC 49417 and 84Pg1) were investigated in dual species approaches with Fusobacterium nucle-atum, Streptococcus oralis, and human gingival fibroblasts. To evaluate a suspected connec-tion with the fimbriae accessory proteins FimCDE, proteomic analysis of outer membrane vesicles (OMVs) was performed. Bundling was confirmed to occur regardless of FimA genotype but is impaired by strong capsule formation. For the first time fimbri-ae-associated OMVs (FAVs) were observed. Tubular and chain-like extensions of the outer membrane (OMEs) were identified in most strains examined, including P. gulae. Bundled fimbriae, FAVs, and OMEs were further suggested to connect to F. nucleatum and S. oralis. Proteome analysis revealed ratios of FimA to accessory proteins to be approximately 13:1 for FimC and FimD and approximately 7:1 for FimE. The results imply more accessory proteins per fimbriae or short FimA fimbriae in OMVs. Since FimCDE is known to be re-sponsible for the adhesion properties and autoaggregation of FimA fimbriae, we propose that it could also mediate the stability of bundled fimbriae and the binding of OMVs to (bundled) fimbriae.
HostingRepository	PRIDE
AnnounceDate	2025-12-30
AnnouncementXML	Submission_2025-12-30_03:42:10.518.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Preisinger
SpeciesList	scientific name: Porphyromonas gingivalis; NCBI TaxID: NEWT:837;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-09 21:19:40	ID requested
⏵ 1	2025-12-30 03:42:11	announced

10.3390/IJMS27010383;

submitter keyword: Porphyromonas gingivalis

Prof. Dr. Georg Conrads
contact affiliation	Division of Oral Microbiology and Immunology, Department of Operative Dentistry, Periodontology and Preventive Dentistry, Rheinisch-Westfälische Technische Hochschule (RWTH) University Hospital, Aa-chen, Germany
contact email	gconrads@ukaachen.de
lab head
Christian Preisinger
contact affiliation	Proteomics Facility, IZKF Aachen, University Clinic RWTH Aachen
contact email	cpreisinger@ukaachen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD070506

PRIDE project URI

• PRIDE
  1. PXD070506
     1. Label: PRIDE project
     2. Name: Porphyromonas gingivalis bundled fimbriae interact with outer membrane vesicles, commensals and fibroblasts