PXD070495-1

PXD070495 is an original dataset announced via ProteomeXchange.

Title	Deep visual proteomics uncovers nociceptor diversity and pain targets
Description	The richness of our somatosensory experience is reflected in the functional diversity of somatic sensory neurons. Single-cell sequencing (scRNA) of sensory neurons has revealed a molecular basis for such diversity1–3. However, sensory neuron diversity has yet to be captured at the level of the proteome. Here, we combined electrophysiology with deep visual proteomics (DVP)4 to quantify over 6,000 proteins from phenotypically-defined sensory neurons in mice and identified proteomic markers of sensory neuron subtypes. Comparative analysis revealed both concordance and meaningful divergence between transcriptomes and proteomes. We further show that up to 3,000 proteins can be quantified from one-fourth of a single neuron, demonstrating subset-specific protein signatures. In culture, nociceptive neurons can be acutely sensitized to mechanical stimuli by nerve growth factor (NGF) which normally drives inflam-matory pain in vivo5. Indeed, overnight exposure of peptidergic nociceptors to NGF and a pro-tein kinase C (PKC) activator produced functional sensitization associated with proteome changes. Functional knockdown experiments identified the up-regulated B3GNT2 enzyme as a potential effector of nociceptor sensitization. In summary, we present a high-resolution proteomic resource linking molecular identity to function, enabling the discovery of mechanisms un-derlying somatic sensation and pain sensitization.
HostingRepository	PRIDE
AnnounceDate	2026-04-11
AnnouncementXML	Submission_2026-04-10_21:23:43.236.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabian Coscia
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF SCP; timsTOF Ultra

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-09 00:13:57	ID requested
⏵ 1	2026-04-10 21:23:43	announced

10.1038/S41467-026-71418-8;

submitter keyword: None

Fabian Coscia
contact affiliation	Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Spatial Proteomics Group, Berlin, Germany (lab head)
contact email	fabian.coscia@mdc-berlin.de
lab head
Fabian Coscia
contact affiliation	Max Delbrück Center
contact email	fabian.coscia@mdc-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD070495

PRIDE project URI

• PRIDE
  1. PXD070495
     1. Label: PRIDE project
     2. Name: Deep visual proteomics uncovers nociceptor diversity and pain targets