PXD070460-1

PXD070460 is an original dataset announced via ProteomeXchange.

Title	Host-to-Pathogen Transfer of Neutrophil Components via Extracellular Vesicles Shields Candida albicans from Immune Attack in Human Blood
Description	Neutrophils effectively eliminate Candida albicans from human blood, but a subset of C. albicans escapes their activity and remains extracellular and viable. Here we show that this is independent from known immune-escape traits of this fungal pathogen. Instead, neutrophil-derived extracellular vesicles (EVs) enriched in antimicrobial proteins and neutrophil surface markers (CD66b, CD11b, CD45, CD63; CR1/3/4 components) promote this state. EVs bind to C. albicans in a complement-dependent manner that required prior C3b/iC3b deposition and was partially blocked by anti-CD11b, consistent with CR3 engagement. Despite their antimicrobial cargo, EVs did not impair fungal growth. Instead, EV coating reduced neutrophil phagocytosis in both purified-cell and whole-blood settings. These findings reveal a dual role for neutrophil EVs at the host-pathogen interface: although enriched for innate effector molecules, their deposition on C. albicans does not impair growth but is associated with reduced phagocytic uptake and maintenance of an extracellular population.
HostingRepository	PRIDE
AnnounceDate	2026-09-04
AnnouncementXML	Submission_2026-09-04_01:47:19.551.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD070460
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thomas Krüger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-07 07:21:55	ID requested
⏵ 1	2026-09-04 01:47:20	announced

Patitz JJ, Nieuwenhuizen NE, Solomatina A, Bachelot Y, Kr, ü, ger T, Svensson CM, Hoeppener S, Zimmermann AK, Blango MG, Martin R, Kniemeyer O, Lange T, Brakhage AA, Figge MT, Kurzai O, H, ü, nniger-Ast K, Host-to-Pathogen Transfer of Neutrophil Components via Extracellular Vesicles Shields Candida albicans From Immune Attack in Human Blood. J Extracell Vesicles, 15(9):e70363(2026) [pubmed]

10.6019/PXD070460;

10.1002/jev2.70363;

submitter keyword: Candida albicans, Immune evasion, Neutrophil-derived extracellular vesicles (EVs), Bloodstream Infection, Complement-dependent opsonization

Thomas Krüger
contact affiliation	Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology / Hans Knöll Institute Jena (Leibniz-HKI), Jena, Germany
contact email	thomas.krueger@leibniz-hki.de
lab head
Thomas Krüger
contact affiliation	Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact email	thomas.krueger@leibniz-hki.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070460
     1. Label: PRIDE project
     2. Name: Host-to-Pathogen Transfer of Neutrophil Components via Extracellular Vesicles Shields Candida albicans from Immune Attack in Human Blood