PXD070408 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Optimization of Protein UFMylation Modification Method and Its Application in Substrate Identification in Human Cells |
| Description | In this study, we generated the UFSP1KO/UFSP2KO HEK293T cell line and validated it as the most suitable cellular model for the screening, identification, and confirmation of UFMylated substrate proteins. Furthermore, exogenous expression of the E3 ligase components UFL1 and DDRGK1 significantly enhances protein UFMylation levels, enabling the large-scale enrichment of UFMylated substrates. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-30 |
| AnnouncementXML | Submission_2026-03-29_16:19:21.105.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zhaopeng Lv |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-06 07:12:43 | ID requested | |
| ⏵ 1 | 2026-03-29 16:19:22 | announced | |
Publication List
| Fang Y, Wang X, Dong J, Yi X, Lv Z, Bu C, Ma X, Cong YS, Liang Q, Optimization of protein UFMylation modification method and its application in substrate identification in human cells. J Biol Chem, 302(4):111320(2026) [pubmed] |
| 10.1016/j.jbc.2026.111320; |
Keyword List
| submitter keyword: UFSP,UFMylation, UFL1, DDRGK1, Substrate |
Contact List
| Qian Liang |
|---|
| contact affiliation | Zhejiang Key laboratory of Medical Epigenetics, School of Basic Medical Sciences, Hangzhou Normal University, Hangzhou, 311121, China |
| contact email | lq@hznu.edu.cn |
| lab head | |
| Zhaopeng Lv |
|---|
| contact affiliation | Micro Bio |
| contact email | lvzhaopenglv@126.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD070408 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD070408
- Label: PRIDE project
- Name: Optimization of Protein UFMylation Modification Method and Its Application in Substrate Identification in Human Cells
