PXD070378 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proximity labeling on phosphatidic acid-enriched membranes generated via optogenetic membrane editing |
| Description | Cellular lipid metabolism is subject to strong homeostatic regulation, but players involved in and mechanisms underlying these pathways remain mostly uncharacterized. Here, we develop and exploit a “Feeding-Fishing” approach coupling membrane editing using optogenetic lipid-modifying enzymes (feeding) with organelle membrane proteomics via proximity labeling (fishing) to elucidate molecular players and pathways involved in homeostasis of phosphatidic acid (PA), a multifunctional lipid central to glycerolipid metabolism. By performing proximity biotinylation using a membrane tethered TurboID alongside membrane editing to selectively deliver PA to the same membrane, we identified numerous PA-metabolizing enzymes and lipid transfer proteins enriched in and depleted from PA-fed membranes. Subsequent mechanistic analysis revealed that PA homeostasis in the cytosolic leaflets of the plasma membrane and lysosomes is mediated by both local PA metabolism and the action of members of the lipid transfer protein families that carry out interorganelle lipid transport prior to additional metabolic steps. More broadly, the interfacing of membrane editing with organelle membrane proteomics using proximity labeling represents a strategy for revealing mechanisms governing lipid homeostasis. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-23 |
| AnnouncementXML | Submission_2026-02-22_17:09:40.631.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Qin Fu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-11-05 13:57:28 | ID requested | |
| ⏵ 1 | 2026-02-22 17:09:41 | announced | |
Publication List
| 10.1038/s41589-025-02104-x; |
| Tei R, Li XL, Luan L, Baskin JM, Membrane editing with proximity labeling reveals regulators of lipid homeostasis. Nat Chem Biol, ():(2026) [pubmed] |
Keyword List
| submitter keyword: Feeding-Fishing, membrane-TurboID, optoPLD, membrane editing, phospholipase D,Phosphatidic acid |
Contact List
| Qin Fu |
|---|
| contact affiliation | Cornell University |
| contact email | qf44@cornell.edu |
| lab head | |
| Qin Fu |
|---|
| contact affiliation | Cornell University |
| contact email | qf44@cornell.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD070378 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD070378
- Label: PRIDE project
- Name: Proximity labeling on phosphatidic acid-enriched membranes generated via optogenetic membrane editing
