PXD070306-1

PXD070306 is an original dataset announced via ProteomeXchange.

Title	The bile acid-CoA ligase, FATP5, is necessary for synthesis of N-acyl taurines in the liver
Description	N-acyl taurines (NAT) are endogenous, bioactive conjugates of fatty acids and taurine with roles in glucose and lipid metabolism. In the liver, NATs share the taurine conjugation enzyme, bile acid-CoA:amino acid N-acyltransferase (BAAT), with bile acids, suggesting the potential of an overlapping hepatic synthesis pathway. However, the remaining enzymes involved in NAT production in the liver remain unknown. This study aimed to investigate the overlap between hepatic NAT and bile acid synthesis to identify additional enzymes involved in NAT synthesis in the liver. Using transcriptomics of livers with altered NAT metabolism, we identified Slc27a5 which encodes fatty acid transport protein 5 (FATP5), as a potential node in the pathway. Knocking down the enzyme using siRNA in vivo confirmed that FATP5 is necessary for hepatic NAT synthesis and upstream of BAAT, likely through its acyl-CoA synthetase activity. The dual functions of this enzyme in activating both fatty acids and bile acids for conjugation implies functional overlap between the hepatic NAT and bile acid production pathway.
HostingRepository	PRIDE
AnnounceDate	2026-02-27
AnnouncementXML	Submission_2026-02-27_15:52:17.950.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivan Bradić
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-04 11:38:01	ID requested
⏵ 1	2026-02-27 15:52:18	announced

Dataset with its publication pending

submitter keyword: NAT, Bile acids and salts/Biosynthesis, SLC27A5, Bile acids and salts/metabolism, Lipids, Fatty Acid/Transport, Liver

Trisha Jean Grevengoed
contact affiliation	Department of Biomedical Sciences University of Copenhagen Denmark
contact email	grevengoed@sund.ku.dk
lab head
Ivan Bradić
contact affiliation	University of Southern Denmark
contact email	ibradic95@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070306
     1. Label: PRIDE project
     2. Name: The bile acid-CoA ligase, FATP5, is necessary for synthesis of N-acyl taurines in the liver