PXD070277-1

PXD070277 is an original dataset announced via ProteomeXchange.

Title	Recoding multiple rare codons enables the simultaneous incorporation of up to five distinct noncanonical amino acids
Description	Expanding the genetic code has revolutionized our ability to study and manipulate biological systems through site-specific incorporation of noncanonical amino acids (ncAAs). However, current methods are primarily limited to single-type ncAA incorporation in mammalian cells owing to translation inefficiency. Here, we introduce a multi-type rare codon recoding strategy that addresses this limitation. By systematically evaluating and repurposing rare codons, alongside engineering mutually orthogonal aminoacyl-tRNA synthetase (aaRS)/tRNA pairs, we achieve the expression of proteins containing two or three distinct ncAAs at site-specific positions with recoding rates of up to 90% at wild-type protein expression levels in mammalian cells. This approach facilitates a broad range of applications, including dual bioorthogonal labeling and sequential protein activation. We further demonstrate the utility of this strategy by incorporating up to five distinct ncAAs into a single protein, revealing a redefinable nature of the genetic code and opening unprecedented avenues for future applications in biomedicine and synthetic biology.
HostingRepository	iProX
AnnounceDate	2025-11-04
AnnouncementXML	Submission_2025-11-03_23:07:14.665.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lihui Lao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-03 23:06:58	ID requested
⏵ 1	2025-11-03 23:07:15	announced

Dataset with its publication pending

submitter keyword: rare codon recoding, multiple rare codon

Shixian Lin
contact affiliation	Zhejiang University
contact email	sxlin@zju.edu.cn
lab head
Lihui Lao
contact affiliation	Zhejiang University
contact email	lihuilao@zju.edu.cn
dataset submitter

iProX dataset URI