PXD070262-1

PXD070262 is an original dataset announced via ProteomeXchange.

Title	Proteome profiling of rabies-infected and uninfected dog brain tissues, cerebrospinal fluids and serum samples
Description	This study aimed to identify potential protein biomarkers for antemortem diagnosis of rabies in dogs, which are the principal reservoir hosts of the rabies virus. Lyssavirus rabies, the prototype species, effectively evades the host immune response allowing the infection to progress unnoticed until the onset of clinical signs. At this stage, the disease is irreversible and invariably fatal, with definitive diagnosis possible only post-mortem. Two hundred and thirty-one samples of brain tissue, cerebrospinal fluid, and serum were collected from apparently healthy dogs brought for slaughter for human consumption in South-East and North-Central Nigeria. All the brain tissue were subjected to a direct fluorescent antibody test to confirm the presence of lyssavirus antigen, and 8.7% (n = 20) were positive. Protein extraction and quantification was performed on 20 rabies-infected and -uninfected samples from each sample type, and only 10 rabies-infected and -uninfected samples were selected from each sample type as they had sufficient protein quantity for further sample preparation. Reduction and alkylation of the selected samples was performed and on-bead HILIC sample clean-up and tryptic digestion followed thereafter. The resulting peptides from each sample were injected into the EvoSep One LC system, coupled to the timsTOF HT mass spectrometer, using the standard dia-PASEF short gradient data acquisition method. Data was processed using Spectronaut (v19) and an unpaired t-test was performed to compare identified protein groups (proteins and their isoforms) between the rabies-infected and uninfected brain tissue, cerebrospinal fluid, and serum samples.
HostingRepository	PRIDE
AnnounceDate	2026-01-05
AnnouncementXML	Submission_2026-01-04_19:59:03.056.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ukamaka Eze
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: NEWT:9615;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-03 17:32:58	ID requested
⏵ 1	2026-01-04 19:59:04	announced

10.3390/proteomes13040066;

Eze UU, Mokoena R, Ogbu KI, Dubazana S, Ngoepe EC, Munangatire M, Ezeokonkwo RC, Anene BM, Buthelezi SG, Sabeta CT, Proteome Profiling of Rabies-Infected and Uninfected Dog Brain Tissues, Cerebrospinal Fluids and Serum Samples. Proteomes, 13(4):(2025) [pubmed]

submitter keyword: proteome profiling, dogs,Rabies, LC-MS/MS analysis, antemortem diagnosis

Rethabile Mokoena
contact affiliation	Proteomics Facility, Council of Scientific and Industrial Research (CSIR), Pretoria, South Africa
contact email	rmokoena1@csir.co.za
lab head
Ukamaka Eze
contact affiliation	University of Nigeria
contact email	ukamakauchenna.eze@unn.edu.ng
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD070262

PRIDE project URI

• PRIDE
  1. PXD070262
     1. Label: PRIDE project
     2. Name: Proteome profiling of rabies-infected and uninfected dog brain tissues, cerebrospinal fluids and serum samples