PXD070241-1

PXD070241 is an original dataset announced via ProteomeXchange.

Title	Defining the Human Lung Pathodegradome of the V8 Protease from Staphylococcus aureus
Description	Staphylococcus aureus is a globally relevant human pathogen and key driver of human morbidity and mortality. During infection it employs a suite of extracellular proteases to subvert host defenses and promote disease progression. Key amongst these, the V8 serine protease (SspA), stands out as a master manipulator of the immune response, however host targets for this enzyme remain incompletely defined. Herein we use a high efficiency N-Terminomic approach developed by our group (TAGS-CR), to capture ~320 biologically relevant V8 targets in the human lung proteome. These substrates present a role for V8 in modulating the complement system, with specific targets like Complement C3 and Complement C5 carrying significant importance in this niche. In addition, neutrophil effector functions such as ROS production, migration, phagocytosis, and degranulation were disrupted by the action of V8. Similarly, epithelial barrier integrity may suffer as a consequence of V8 targeting foundational proteins such as zona occludens and alpha actinin proteins; coupled with supporting actin molecules. Notably, our data provides new insights into V8 as a key player exploiting nutritional immunity by disrupting iron-complexed proteins such as transferrins, hemopexin and hemoglobin. In addition to its capacity to intensify inflammation, V8 also undermines the function of key immune regulators, including pro-inflammatory sink molecules like gelsolin. Collectively, this comprehensive proteolytic mapping expands our understanding of how S. aureus can manipulate human biology across infection relevant niches and offers valuable insight into conserved molecular mechanisms that underlie bacterial virulence.
HostingRepository	PRIDE
AnnounceDate	2026-02-03
AnnouncementXML	Submission_2026-02-03_03:12:31.137.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emilee Mustor
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	L-homoarginine
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-03 12:11:21	ID requested
⏵ 1	2026-02-03 03:12:31	announced

10.1128/IAI.00725-25;

submitter keyword: Degradomics, V8 Protease, TAGS-CR,Staphylococcus aureus

Lindsey Shaw
contact affiliation	Department of Molecular Biosciences, University of South Florida, Tampa, FL, USA Center for Antimicrobial Resistance, University of South Florida, Tampa, FL, USA
contact email	shaw@usf.edu
lab head
Emilee Mustor
contact affiliation	University of South Florida
contact email	emileemustor@usf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD070241

PRIDE project URI

• PRIDE
  1. PXD070241
     1. Label: PRIDE project
     2. Name: Defining the Human Lung Pathodegradome of the V8 Protease from Staphylococcus aureus