PXD070221-1

PXD070221 is an original dataset announced via ProteomeXchange.

Title	Proteomic validation of MEG-01-Derived Extracellular Vesicles as Biologically Representative Models for Megakaryocyte- and Platelet-Derived Extracellular Vesicles.
Description	Platelets and their extracellular vesicles (EVs) have emerged as promising liquid biopsy biosources for cancer detection and monitoring. The megakaryoblastic MEG-01 cell line offers a controlled system for generating platelet-like particles (PLPs) and EVs through valproic acid induced differentiation. Here, we performed comprehensive characterization and proteomic validation of MEG-01-derived populations, native human platelets and their EVs using nanoparticle tracking analysis, transmission electron microscopy, imaging flow cytometry and quantitative proteomics. MEG-01 megakaryocytic differentiation is characterized by polylobulated nuclei, proplatelet formation and elevated CD41/CD42a expression. PLPs predominantly exhibit an activated-like phenotype (CD62P+, degranulated morphology), while microvesicles (100-500 nm) and exosomes (50-250 nm) displayed size distributions and phenotypic markers consistent with native platelet-derived EVs. Proteomics identified substantial core proteomes shared across fractions and fraction-specific patterns consistent with selective cargo partitioning during EV biogenesis. Functional enrichment revealed that MEG-01-derived vesicles retained hemostatic, cytoskeletal and immune pathways characteristic of physiological platelet EVs. Ingenuity Pathway Analysis demonstrated that PLPs maintained proliferative transcriptional programs (elevated MYC/RB1/TEAD1, reduced GATA1), while plasma exosomes showed minimal differential pathway activation relative to MEG-01 exosomes. These findings validate MEG-01-derived EVs as representative of megakaryocyte-lineage exosomes and activated platelet-like states; plasma exosomes converge proteomically with EXOs MEG-01, whereas platelet exosomes retain distinct activation-associated features.
HostingRepository	PRIDE
AnnounceDate	2026-01-05
AnnouncementXML	Submission_2026-01-05_00:27:45.291.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carolina Torres Perales
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-03 02:51:16	ID requested
⏵ 1	2026-01-05 00:27:46	announced

Sanchez-Manas JM, Perales S, Martinez-Navajas G, Ceron-Hernandez J, Lopez CM, Peralbo-Molina A, Delgado JR, Martinez-Galan J, Ramos-Mejia V, Chicano-Galvez E, Hernandez-Valladares M, Ortuno FM, Torres C, Real PJ, Proteomic Validation of MEG-01-Derived Extracellular Vesicles as Representative Models for Megakaryocyte- and Platelet-Derived Extracellular Vesicles. Biomolecules, 15(12):(2025) [pubmed]

10.3390/biom15121698;

submitter keyword: MEG-01 cell line, Exosomes (EXOs)., Extracellular Vesicles (EVs), Proteomics, Microvesicles (MVs), Platelets

Pedro J. Real
contact affiliation	1. Gene Regulation, Stem Cells and Development group, PTS, Granada GENyO, Pfizer-University of Granada-Andalusian Regional Government Centre for Genomics and Oncological Research, Avenida de la Ilustración 114, 18016 Granada, Spain. 2. Department of Biochemistry and Molecular Biology I, Faculty of Science, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain. 3. Instituto de Investigación Biosanitaria ibs.GRANADA, Granada, Spain.
contact email	pedro.real@genyo.es
lab head
Carolina Torres Perales
contact affiliation	Department of Biochemistry and Molecular Biology III and Immunology, University of Granada
contact email	ctp@ugr.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD070221

PRIDE project URI

• PRIDE
  1. PXD070221
     1. Label: PRIDE project
     2. Name: Proteomic validation of MEG-01-Derived Extracellular Vesicles as Biologically Representative Models for Megakaryocyte- and Platelet-Derived Extracellular Vesicles.