PXD070203-1

PXD070203 is an original dataset announced via ProteomeXchange.

Title	Quantitative 4D-DIA LC–MS/MS analysis of TPM3P9 RNA pulldown proteins in primary breast cancer cells (PBCs#2/#3/#5) and bone-metastatic breast cancer cells (BMBCs#2/#3/#5)
Description	This project aims to identify and quantify TPM3P9 RNA-associated proteins in primary breast cancer cells (PBCs#2/#3/#5) and bone-metastatic breast cancer cells (BMBCs#2/#3/#5) using 4D-DIA LC–MS/MS. RNA pulldown assays were performed with tRSA-based TPM3P9 RNA to capture interacting proteins, which were subsequently analyzed by 4D-DIA-based quantitative proteomics. The objective is to elucidate the translational regulation mechanism of osteolysin in bone-metastatic breast cancer cells and to identify specific RNA-binding proteins involved in osteolysin translation.
HostingRepository	iProX
AnnounceDate	2025-10-31
AnnouncementXML	Submission_2025-11-03_00:12:03.079.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuxia Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-03 00:11:23	ID requested
⏵ 1	2025-11-03 00:12:03	announced

Dataset with its publication pending

submitter keyword: 4D-DIA, LC-MS/MS, TPM3P9, tRSA RNA pulldown, primary breast cancer cells, osteolysin, RNA-binding proteins, RBM46, translation regulation

Jun Li
contact affiliation	Sun Yat-sen University
contact email	lijun37@mail.sysu.edu.cn
lab head
Shuxia Zhang
contact affiliation	Sun Yat-sen University
contact email	zhangshuxia1993@126.com
dataset submitter

iProX dataset URI