PXD070162-1

PXD070162 is an original dataset announced via ProteomeXchange.

Title	Unique shaping of the chloroplastic iron-sulfur transfer protein NFU1 sustains dark chlorophyll synthesis and fermentation in Chlamydomonas reinhardtii
Description	The sulfur mobilization (SUF) machinery is required for the synthesis of iron-sulfur (Fe- S) clusters and their insertion into client proteins in plastids. The final step relies on several Fe-S cluster transfer proteins, including NIFU-like (NFU) proteins. In this study, we focused on the chloroplastic elongated NFU1 from the green microalga Chlamydomonas reinhardtii. It possesses an N-terminal putative endonuclease domain present in orthologs from many eukaryotic photosynthetic organisms but not from Angiosperms. Using a reverse genetic approach, we demonstrated that CrNFU1 serves as the major maturation factor for several [4Fe-4S]-cluster containing proteins involved in specialized pathways. This includes an atypical hybrid cluster protein and an iron-iron hydrogenase operating in anoxia, as well as the dark-operative protochlorophyllide a oxidoreductase (DPOR) involved in chlorophyll synthesis in the dark. While the N-terminal domain has no in vitro DNA endonuclease activity, it is crucial for CrNFU1 function. Considering that CrNFU1 has retained the capacity to bind nucleic acids and was found associated with chloroplast ribosomes, we propose that the N-terminal domain of CrNFU1 contributes to the co-translational insertion of the [4Fe-4S] cluster(s) in DPOR subunits which are coded by the chloroplast genome. The strong co-occurrence between elongated NFU1 representatives and DPOR supports this intertwined function.
HostingRepository	PRIDE
AnnounceDate	2026-07-07
AnnouncementXML	Submission_2026-07-07_03:24:52.270.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD070162
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Didier Vertommen
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: NEWT:3055;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-31 10:09:33	ID requested
⏵ 1	2026-07-07 03:24:53	announced

Przybyla-Toscano J, Kairis A, Terenzi A, Caccamo A, Ruys SPD, Vertommen D, Rouhier N, Remacle C, The chloroplastic NFU1 maturation factor sustains iron-sulfur cluster assembly in the dark in Chlamydomonas. Plant Physiol, ():(2026) [pubmed]

10.6019/PXD070162;

10.1093/plphys/kiag466;

submitter keyword: iron-sulfur cluster

transfer protein, endonuclease domain

GYI-YIG motif

NFU domains

green microalga

chloroplast

Claire Remacle
contact affiliation	University of Liège, Genetics and Physiology of Microalgae, InBios/Phytosystems research unit, 4000 Liège, Belgium
contact email	c.remacle@uliege.be
lab head
Didier Vertommen
contact affiliation	UCL - de Duve Institute, Brussels Belgium
contact email	didier.vertommen@uclouvain.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070162
     1. Label: PRIDE project
     2. Name: Unique shaping of the chloroplastic iron-sulfur transfer protein NFU1 sustains dark chlorophyll synthesis and fermentation in Chlamydomonas reinhardtii