PXD070116-1

PXD070116 is an original dataset announced via ProteomeXchange.

Title	Multi-omics analysis of arboviral capsid targets in mosquitoes reveals a pro-viral function of the chromatin-remodeling Brahma complex
Description	In recent years, arboviral infections have surged dramatically due to the geographic expansion of Aedes and Culex mosquitoes, their main vector mosquitoes. Despite significant efforts to uncover arbovirus–host interactions and viral protein effector functions in mammals, systematic studies aiming to characterize virus–vector interactions in arthropods are largely missing, and the functions and cellular targets of many arboviral proteins in mosquitoes remain elusive. Here, we applied a multi-omic approach to systematically evaluate the ability of arboviral capsids to interact with the Ae. aegypti proteome. This extensive multi-modal atlas across 12 pathogenic arboviral species spanning three viral genera revealed shared and distinct host factor specificities, uncovering species-, genus- and vector preference-specific patterns of host usage in mosquitoes. Functional phenotypic screening of 110 newly discovered host proteins across three prototypic arboviruses (La Crosse virus, dengue virus and West Nile virus) identified several novel host dependency factors, including a new role for the chromatin-remodeling Brahma complex in orthoflavivirus replication. Using a combination of biochemical and sequencing approaches, we characterized the cellular determinants of these interactions and profiled their functional consequences on the chromatin landscape. Altogether, this study provides a multi-layered repository to categorize and characterize arboviral capsid effector functions in invertebrates, providing important cues on novel mechanisms of transcriptional regulation via capsid-mediated modulation of chromatin accessibility in insects.
HostingRepository	PRIDE
AnnounceDate	2026-02-05
AnnouncementXML	Submission_2026-02-05_04:29:45.222.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pietro Scaturro
SpeciesList	scientific name: Aedes aegypti; NCBI TaxID: NEWT:7159;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-30 14:46:19	ID requested
⏵ 1	2026-02-05 04:29:45	announced

10.1016/J.MCPRO.2026.101512;

submitter keyword: None

Pietro Scaturro
contact affiliation	Systems Arbovirology, Leibniz Institute of Virology (LIV), Hamburg, Germany
contact email	pietro.scaturro@leibniz-liv.de
lab head
Pietro Scaturro
contact affiliation	Systems Arbovirology, Leibniz Institute of Virology
contact email	pietro.scaturro@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD070116

PRIDE project URI

• PRIDE
  1. PXD070116
     1. Label: PRIDE project
     2. Name: Multi-omics analysis of arboviral capsid targets in mosquitoes reveals a pro-viral function of the chromatin-remodeling Brahma complex