PXD070089-1

PXD070089 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Prostate Cancer Tissue Small Extracellular Vesicles Reveal Alteration of Metabolism
Description	Prostate cancer (PCa) is a leading male malignancy worldwide, with metabolic reprogramming being a critical hallmark of its progression. Extracellular vesicles (EVs) derived from tissues directly reflect the tumor microenvironment, offering unique insights into cancer pathophysiology that are unattainable through cell line or biofluid-derived EVs. However, the functional roles of tissue-derived EVs in PCa metabolism remain poorly understood. Leveraging our expertise in murine PCa model establishment and EV isolation from prostate tissue, this study aimed to characterize functional differences between PCa and normal prostate tissue via proteomic analysis of tissue-derived small EVs (sEVs). We orthotopically implanted luciferase-labeled PCa cells into nude mice to establish an in situ PCa model, confirmed tumor formation via in vivo imaging, and harvested tissues after four weeks. sEVs were isolated using ultracentrifugation combined with an iodixanol density cushion and characterized by transmission electron microscopy, nanoparticle tracking analysis, and protein marker profiling. Proteomic analysis identified 28 upregulated and 24 downregulated proteins in PCa-derived sEVs compared to normal controls. Subcellular localization revealed enrichment in the cytoplasm, while pathway analysis highlighted significant involvement in metabolic processes, particularly glycolysis, amino acid biogenesis, carbon metabolism, and pyruvate metabolism. Our study establishes a robust method for isolating prostate tissue sEVs and provides the first evidence that PCa tissue-derived sEVs exhibit profound metabolic pathway alterations. These findings enhance our understanding of PCa progression mechanisms and may facilitate the development of novel diagnostic biomarkers and therapeutic strategies targeting metabolic dysregulation in PCa.
HostingRepository	PRIDE
AnnounceDate	2026-03-16
AnnouncementXML	Submission_2026-03-16_03:49:33.282.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ting Ding
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-30 04:36:20	ID requested
⏵ 1	2026-03-16 03:49:34	announced

10.1002/pmic.70081;

Yu L, Ding T, Olofsson Bagge R, Zheng L, Hao X, Proteomics of Prostate Cancer Tissue Small Extracellular Vesicles Reveal Alteration of Metabolism. Proteomics, 26(2-3):121-128(2026) [pubmed]

submitter keyword: tumor metabolism,Prostate cancer, tissue EVs, Glycolysis

Lijuan Yu
contact affiliation	Sahlgrenska Center for Cancer Research and Wallenberg Centre for Molecular and Translational Medicine, Department of Surgery, Institute of Clinical Sciences, University of Gothenburg, Gothenburg, 40530, Sweden;
contact email	lijuan.yu@gu.se
lab head
Ting Ding
contact affiliation	The Second Hospital Affiliated to Xi’an Jiaotong University
contact email	dr_dingting@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD070089

PRIDE project URI

• PRIDE
  1. PXD070089
     1. Label: PRIDE project
     2. Name: Proteomics of Prostate Cancer Tissue Small Extracellular Vesicles Reveal Alteration of Metabolism