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PXD070017-1
PXD070017 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Chemoproteomics reveals extensive cysteine S-sulfhydration in deep-sea hyperthermophilic archaea |
| Description | Thermococcales are an order of archaea that inhabit the sulfur-rich, anaerobic environments of deep-sea hydrothermal vents. Their metabolism shifts toward the production of hydrogen sulfide (H₂S) in the presence of elemental sulfur. As a gaseous signaling molecule, H₂S primarily regulates biological processes by mediating the S-sulfhydration of protein cysteine. However, it remains unknown whether such modifications occur in these archaea. In this study, we employed chemical proteomics to systematically identify S-sulfhydration sites in the deep-sea hyperthermophilic archaeon Thermococcus aciditolerans SY113. This approach revealed unique reactivity characteristics of the modification and allowed quantitative analysis of its dynamic regulation by H₂S. A total of 204 S-sulfhydration sites on 171 proteins were identified, over 65% of which were dynamically regulated by H₂S. Further functional analysis indicated that S-sulfhydration plays an ancient and conserved role in primordial cells, including the regulation of catalytic activity, maintenance of protein conformation, and mediation of protein-protein interactions. Our findings provide a valuable dataset and theoretical foundation for understanding the role of S-sulfhydration in the physiological regulation of deep-sea hyperthermophilic archaea. |
| HostingRepository | iProX |
| AnnounceDate | 2025-10-29 |
| AnnouncementXML | Submission_2025-12-31_00:25:05.199.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ling Fu |
| SpeciesList | scientific name: Thermococcus eurythermalis; NCBI TaxID: 1505907; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-29 01:54:58 | ID requested | |
| ⏵ 1 | 2025-12-31 00:25:05 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Archaea, Thermococcales, H₂S, S-sulfhydration, chemical proteomics, GAP, SAT, TFIIB |
Contact List
| Jing Yang | |
|---|---|
| contact affiliation | Beijing Institute of Lifeomics |
| contact email | yangjing@ncpsb.org.cn |
| lab head | |
| Ling Fu | |
| contact affiliation | Beijing Institute of Lifeomics |
| contact email | flsmt@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
