PXD069999-1

PXD069999 is an original dataset announced via ProteomeXchange.

Title	Coordinated regulation of metabolic plasticity by the Hsp90 chaperones
Description	Heat shock protein 90 (Hsp90) chaperones participate in the stabilization and activation of hundreds of proteins, thereby acting as signaling hubs. A mitochondrial subpopulation of Hsp90 has been previously described; however, little is known about its role in metabolism. Here, we showed that loss of individual Hsp90 isoforms differentially affects oxygen consumption and metabolic flexibility. Proteomic and metabolomic evaluation demonstrated that Hsp90 regulates the mitochondrial metabolic network, including respiration, fatty acid oxidation and redox homeostasis. Loss of the mitochondrial chaperone TRAP1 induced compensatory binding of Hsp90s to TRAP1-dependent proteins, indicating a mechanism for the role of Hsp90 chaperones in metabolic reprogramming. When considered with previous findings, a temporal pattern of regulation emerges whereby Hsp90s control the transcription, translation, import, and assembly of mitochondrial protein complexes. Our findings expand the scope of Hsp90-regulated processes and potentially inform the effects of isoform-specific Hsp90 inhibitors on metabolic reprogramming in cancer and other diseases.
HostingRepository	PRIDE
AnnounceDate	2026-05-01
AnnouncementXML	Submission_2026-05-01_07:13:04.995.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Backe
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-28 18:25:29	ID requested
⏵ 1	2026-05-01 07:13:05	announced

Dataset with its publication pending

submitter keyword: Mitochondria, Metabolism, post-translational modification, TRAP1, molecular chaperone, Heat-shock protein, Hsp90

Mark Woodford
contact affiliation	Department of Urology, SUNY Upstate Medical University
contact email	woodform@upstate.edu
lab head
Sarah Backe
contact affiliation	SUNY Upstate Medical University
contact email	backes@upstate.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD069999

PRIDE project URI

• PRIDE
  1. PXD069999
     1. Label: PRIDE project
     2. Name: Coordinated regulation of metabolic plasticity by the Hsp90 chaperones