PXD069984-1

PXD069984 is an original dataset announced via ProteomeXchange.

Title	Assessing inter-line reproducibility of a novel iPSC-derived 3D brain tissue model (3BTM) and comparison to 2D co-cultures
Description	Stem-cell-based in vitro models offer promising potential to elucidate human brain cell functions and interactions under healthy conditions and in disease states. However, harnessing this potential is currently impaired by low reproducibility, maturity, and cell-type diversity of models. Therefore, we developed a novel 3D brain tissue model (3BTMs) containing neurons, astrocytes, and microglia with high reproducibility, maturity, and viability. To confirm reproducibility of culture generation, composition, and maturation across iPSC lines, and compare 3BTMs to 2D co-cultures of the same cells, we performed proteome analysis of 3BTMs from 3 independent wild-type cell lines over time and in comparison to 2D co-cultures, fetal and juvenile brain samples, and neural organoids from 2 independent lines.
HostingRepository	PRIDE
AnnounceDate	2026-02-24
AnnouncementXML	Submission_2026-02-24_03:36:23.895.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-28 07:12:22	ID requested
⏵ 1	2026-02-24 03:36:24	announced

Dataset with its publication pending

submitter keyword: 3D brain tissue model, 3BTM, brain, iPSC

Stefan F. Lichtenthaler
contact affiliation	DZNE Munich, Neuroproteomics, Feodor-Lynen-Str. 17, 81377 München, Germany
contact email	stefan.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD069984

PRIDE project URI

• PRIDE
  1. PXD069984
     1. Label: PRIDE project
     2. Name: Assessing inter-line reproducibility of a novel iPSC-derived 3D brain tissue model (3BTM) and comparison to 2D co-cultures