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PXD069977-1
PXD069977 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Analysis of proteome dynamics in early-stage sporulation-inhibited variants of Parageobacillus thermoglucosidasius |
| Description | Parageobacillus thermoglucosidasius is a thermophilic Gram-positive that is currently on the rise as a potential platform for sustainable chemical production. One major issue with P. thermoglucosidasius as a host is its natural ability to sporulate in response to nutrient starvation, as endospore formation is generally undesired in industrial fermentations. It is therefore of interest to instead employ a sporulation-supressed variant of P. thermoglucosidasius. However, as a non-model species, the complex regulatory system that governs sporulation in P. thermoglucosidasius is currently poorly characterised. This study presents a characterisation and proteomic analysis of the P. thermoglucosidasius wildtype strain alongside four early-stage sporulation-inhibited variants. The genes targeted for sporulation-inhibition were Spo0A, Spo0B, Spo0F, and SigF, based on their regulatory roles in the sporulation pathway of Bacillus subtilis. Proteomics data was collected from four different growth stages and the resulting expression profiles were compared. This led to the identification of a co-expression module consisting of approximately 200 genes, which through enrichment was confirmed to be closely linked to the P. thermoglucosidasius sporulation pathway. The results of the analysis have been made publicly available. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-22 |
| AnnouncementXML | Submission_2025-12-21_17:19:10.245.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD069977 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Oihane Irazoki |
| SpeciesList | scientific name: Parageobacillus thermoglucosidasius NBRC 107763; NCBI TaxID: NEWT:1223501; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-28 04:04:11 | ID requested | |
| ⏵ 1 | 2025-12-21 17:19:11 | announced |
Publication List
| 10.6019/PXD069977; |
| Millgaard M, Irazoki O, Mol V, Pogrebnyakov I, Nielsen AT, . Int J Mol Sci, 26(23):(2025) [pubmed] |
| 10.3390/ijms262311735; |
Keyword List
| submitter keyword: Parageobacillus thermoglucosidasius |
| thermophile |
| sporulation |
| Orbitrap Exploris 480 |
Contact List
| Alex Toftgaard Nielsen | |
|---|---|
| contact affiliation | Bacterial Cell Factories, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark |
| contact email | atn@biosustain.dtu.dk |
| lab head | |
| Oihane Irazoki | |
| contact affiliation | Technical University of Denmark |
| contact email | oihira@biosustain.dtu.dk |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD069977 |
| PRIDE project URI |
Repository Record List
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