⮝ Full datasets listing
PXD069974-1
PXD069974 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A Key Viral Factor for COVID-19 Virulence: SARS-CoV-2 ORF7b Dysregulates the Lysosomal-Autophagy System for Enhanced Replication |
| Description | The pandemic Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is known for its high infectivity, high replication rate, and rapid transmission in humans. However, the key viral factor that determines these features has yet to be identified. In this study, the SARS-CoV-2 ORF7b protein was identified as a key factor promoting viral replication and infectivity, leading to the rapid spread of the virus to neighboring cells. Mechanistically, ORF7b blocks the assembly of the V0 complex of the v-type ATPase proton pump by hijacking and inducing the degradation of V0 complex subunits. This interference prevents the formation of the V0-V1 complex, resulting in lysosomal de-acidification. Furthermore, ORF7b promotes autophagy activation through the GCN2 pathway, which is linked to amino acid deprivation signaling. Pharmacological interventions demonstrated that lysosomal activation via ATPase agonists effectively inhibited viral spread, while inhibiting GCN2-dependent autophagy activation suppressed viral replication. In a mouse model, these pharmacological agents also reduced lung viral replication, inflammation, and mitigated lung damage. These novel findings demonstrate that ORF7b plays a pivotal role in the viral life cycle by dysregulating the host's lysosomal-autophagy system, positioning this host pathway as a potential therapeutic target for COVID-19 treatment. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-04-30 |
| AnnouncementXML | Submission_2026-04-29_18:57:05.761.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hyeong Min Lee |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; TMT; TMT |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-28 02:22:45 | ID requested | |
| ⏵ 1 | 2026-04-29 18:57:06 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: SARS-CoV-2, ORF7b, viral replication, viral transmission, autophagy, lysosomal dysfunction, v-ATPase, GCN2 pathway |
Contact List
| Shin-Gu choul | |
|---|---|
| lab head | |
| Hyeong Min Lee | |
| contact affiliation | Jeju univesity |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004143/ |
