PXD069973-1

PXD069973 is an original dataset announced via ProteomeXchange.

Title	Sacubitril/Valsartan mitigates atrial fibrillation in the rat model by suppressing activation of CaMKII/NF-κB signaling and attenuating inflammation: an ultra-fast quantitative proteomics study
Description	Atrial fibrillation (AF) is one of the most common arrhythmias that significantly impacts the quality of life and prognosis of patients. Sacubitril/valsartan (SV), a commonly prescribed combinatorial medication for heart failure, has shown good clinical efficacy in managing AF, but the underlying mechanisms are not clear. This study used proteomic profiling to investigate the mechanisms underlying the interventional effects of SV using a Sprague-Dawley (SD) rat model of AF. Atrial tissues were obtained from the control, AF model, and SV-treated AF model rats and analyzed by ultra-fast proteomic profiling to identify differentially expressed proteins (DEPs) associated with AF and SV-related effects. Bioinformatics tools and experimental validation by ELISA and western blotting were used to determine the signaling pathways and potential mechanisms underlying AF and SV-related effects based on the proteomics data. Proteomic profiling demonstrated distinct protein expression profiles across the control, AF, and SV-treatment groups. Integrated analysis of the DEPs and experimental validation demonstrated that SV treatment alleviated AF in the rats atrium by suppressing activation of the CaMKII and NF-κB signaling pathway, thereby attenuating inflammatory responses in the atrial tissues. Our findings demonstrate that SV mitigates AF in the rat model by suppressing activation of CaMKII and NF-κB signaling pathway, thereby attenuating inflammation. Therefore, sacubitril/valsartan is a promising treatment for AF, but requires further clinical investigations in humans.
HostingRepository	iProX
AnnounceDate	2025-10-27
AnnouncementXML	Submission_2025-10-28_01:45:18.831.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xia Zhang
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-28 01:45:04	ID requested
⏵ 1	2025-10-28 01:45:19	announced

Dataset with its publication pending

submitter keyword: Atrial fibrillation, Sacubitril/Valsartan;proteomics

Yubing Wang
contact affiliation	Chuanbei Medical College Affiliated Hospital
contact email	495720873@qq.com
lab head
Xia Zhang
contact affiliation	Chuanbei Medical College Affiliated Hospital
contact email	2499021502@qq.com
dataset submitter

iProX dataset URI