PXD069956 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Engineering an Extremely Hybrid PKS for Adipic Acid Production |
| Description | Polyketide synthases (PKSs) are modular enzymes with exceptional potential as biocatalysts for producing non-native compounds. Here, we report the first PKS-based pathway for adipic acid (AA), an industrial monomer for nylon production, by engineering one of the most extensively hybridized PKS systems to date. Using a retrobiosynthetic approach, we identified EtnB, a succinyl-CoA loading module that uniquely retains the terminal carboxyl group, enabling access to dicarboxylic polyketide products, rarely produced by canonical PKSs. EtnB was coupled to a fully reducing extension module through an engineered communication linker, which improved ACP–KS interactions, enhanced titers, and demonstrated selective S-CoA loading in vivo. This construct integrates genes from five organisms, seven PKS modules joined across six non-natural junctions, and functions in both Escherichia coli and Pseudomonas putida. Additional engineering that included AT domain swaps, optimization of extender unit supply, and host strain metabolic rewiring further improved AA titers into the low mg/L range. Together, this work demonstrates that highly chimeric PKSs can be rendered functional through rational design, expands the PKS toolkit with a carboxyl-retaining loading module, and establishes a versatile platform for engineering diacids and other non-canonical products through PKS pathways. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-07 |
| AnnouncementXML | Submission_2026-09-07_04:24:09.310.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Christopher Petzold |
| SpeciesList | scientific name: Pseudomonas putida KT2440; NCBI TaxID: NEWT:160488; |
| ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-27 16:43:09 | ID requested | |
| ⏵ 1 | 2026-09-07 04:24:10 | announced | |
Publication List
| Klass SH, Wang Z, Lanclos N, Wesselkamper M, Li C, Baerwald J, Pearson A, Thomas M, Hu W, Schmidt M, Chen Y, Gin JW, Petzold CJ, Keasling JD, Engineering an Extremely Hybrid PKS for Adipic Acid Production. ACS Synth Biol, 15(7):2887-2899(2026) [pubmed] |
| 10.1021/acssynbio.5c00972; |
Keyword List
| submitter keyword: Hybrid PKS, Polyketide Synthase,Adipic acid, P. putida,JBEI, E. coli |
Contact List
| Christopher Petzold |
|---|
| contact affiliation | Staff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720 |
| contact email | cjpetzold@lbl.gov |
| lab head | |
| Christopher Petzold |
|---|
| contact affiliation | Lawrence Berkeley National Laboratory |
| contact email | cjpetzold@lbl.gov |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD069956 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069956
- Label: PRIDE project
- Name: Engineering an Extremely Hybrid PKS for Adipic Acid Production
