PXD069946 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Size-exclusion chromatography of soluble chloroplast proteins from Nicotiana tabacum (tobacco) |
| Description | Maturase K (MatK) is the only splicing factor encoded in the chloroplast genomes of land plants. Here we used a transplastomic Nicotiana tabacum line, in which the plastidial MatK gene is C-terminally tagged with a 3x-HA tag, to conduct size-exclusion chromatography of soluble protein extracts enriched for chloroplast proteins. Fractions 5 and 6, in which MatK-HA was found most abundant by western blot analysis, were subjected to mass spectrometry analysis. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-10 |
| AnnouncementXML | Submission_2026-02-10_06:17:03.851.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Barbara Pfister |
| SpeciesList | scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: NEWT:4097; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-27 09:18:02 | ID requested | |
| ⏵ 1 | 2026-02-10 06:17:04 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Maturase K,LC-MS/MS, tobacco, DIA, size-exclusion chromatography, chloroplast |
Contact List
| Samuel C. Zeeman |
|---|
| contact affiliation | Institute of Molecular Plant Biology, Department of Biology, ETH Zurich, Switzerland |
| contact email | szeeman@ethz.ch |
| lab head | |
| Barbara Pfister |
|---|
| contact affiliation | ETH Zurich |
| contact email | bpfister@ethz.ch |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069946
- Label: PRIDE project
- Name: Size-exclusion chromatography of soluble chloroplast proteins from Nicotiana tabacum (tobacco)
