PXD069892-1

PXD069892 is an original dataset announced via ProteomeXchange.

Title	Proteomics Analysis of Human Mesenchymal Stromal/Stem Cell Sarcomagenesis Model Identifies ALDH1A3 and CD99 as Potential Targets in the Transformation Process
Description	Mesenchymal stromal/stem cells (MSC) may represent the cell-of-origin for sarcoma development. A collection of human MSCs sequentially mutated with an increasing number of oncogenic hits served to recreate a step-wise process of sarcomagenesis. To identify potential protein targets of interest in the MSC-sarcoma transformation process, quantitative mass spectrometry based (LC-MS/MS) proteomics was performed. Among the proteins hits identified as significantly regulated in the transformation process ALDH1A3 and CD99 were selected and further studied. Both ALDH1A3 abundance levels and activity were significantly up-regulated in early phase (immortalized) and fully transformed (sarcoma forming) cells as compared to normal MSCs. Inversely, CD99 total protein and cell-surface abundance levels were down-regulated in immortalized and transformed MSCs. Down-regulated CD99 was also identified in several human bone and soft tissue sarcoma subtypes. Proteomics investigation of a MSC-transformation model of sarcoma has yielded ALDH1A3 and CD99 as potential targets for sarcomagenesis that may contribute to a greater understanding of the disease and the development of novel therapeutic approaches.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_16:20:42.869.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD069892
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hala Halabi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-24 16:24:43	ID requested
⏵ 1	2026-02-22 16:20:43	announced

10.1186/s12915-025-02498-z;

Gobin JM, Gao J, Rey V, Torn, í, n J, Muradia G, Halabi H, Bueno C, Guerrero-Murillo M, Lopez-Millan B, Menendez P, Rosu-Myles M, Rodriguez R, Lavoie JR, Proteomics analysis of human mesenchymal stromal/stem cell sarcomagenesis model identifies ALDH1A3 and CD99 as potential targets in the transformation process. BMC Biol, 24(1):32(2026) [pubmed]

10.6019/PXD069892;

submitter keyword: MSC-sarcoma model, sarcomagenesis, CD99, proteomics (LC-MS/MS) , ALDH1A3

Jessie R.Lavoie
contact affiliation	Centre for Oncology, Radiopharmaceuticals and Research, Biologic and Radiopharmaceutical Drugs Directorate, Health Products and Food Branch, Health Canada, Ottawa, ON, K1A 0K9, Canada
contact email	jessie.lavoie@hc-sc.gc.ca
lab head
Hala Halabi
contact affiliation	Health Canada
contact email	hala.halabi@hc-sc.gc.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD069892
     1. Label: PRIDE project
     2. Name: Proteomics Analysis of Human Mesenchymal Stromal/Stem Cell Sarcomagenesis Model Identifies ALDH1A3 and CD99 as Potential Targets in the Transformation Process