PXD069882 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | C2C12 PEA and Ibuprofen LC-MS/MS |
| Description | This study investigated the effects of palmitoylethanolamide (PEA) and ibuprofen (IBU) treatment on the dynamic regulation of the skeletal muscle proteome using a C2C12 myotube model. Differentiated myotubes were treated for 36 hours with vehicle control (VC), PEA (10 µM), or IBU (100 µM). Dynamic proteome profiling was performed using deuterium oxide (D₂O) metabolic labelling combined with high-resolution LC–MS/MS analysis on an Orbitrap Q-Exactive (QE) instrument. Samples were collected at 0 h, 12 h, 24 h, and 36 h, with n = 3 biological replicates per timepoint and condition. Protein-specific fractional synthesis rates (FSR) were derived from the 12–36 h labelling period, and relative protein abundance was quantified at 36 h. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-25 |
| AnnouncementXML | Submission_2026-05-24_23:03:05.711.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD069882 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Connor Stead |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-10-24 08:06:42 | ID requested | |
| ⏵ 1 | 2026-05-24 23:03:06 | announced | |
Publication List
| 10.6019/PXD069882; |
| 10.1096/fba.2025-00286; |
| Cole PL, Stead CA, Burniston JG, Owens DJ, The Effects of Palmitoylethanolamide or Ibuprofen on the Abundance Profile and Synthesis Rate of Proteins in C2C12 Skeletal Myotubes. FASEB Bioadv, 8(4):e70108(2026) [pubmed] |
Keyword List
| submitter keyword: C2C12, Muscle, LCMS/MS |
Contact List
| Dan Owens |
|---|
| contact affiliation | Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, United Kingdom |
| contact email | d.j.owens@ljmu.ac.uk |
| lab head | |
| Connor Stead |
|---|
| contact affiliation | Liverpool John Moores University |
| contact email | c.stead@ljmu.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD069882 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD069882
- Label: PRIDE project
- Name: C2C12 PEA and Ibuprofen LC-MS/MS
